. . . "The role of genome and oxygen radicals"@en . "20"^^ . . . . . "20"^^ . "0"^^ . "Zam\u00FD\u0161l\u00EDme pou\u017E\u00EDt kvasinky a sav\u010D\u00ED o\u010Dn\u00ED \u010Do\u010Dky jako model pro studium proces\u016F st\u00E1rnut\u00ED. Tyto dva paraleln\u00ED modely jsou vhodn\u00E9 pro porovn\u00E1vac\u00ED studia p\u0159i studiu st\u00E1rnut\u00ED, p\u0159edstavuj\u00ED sice dva r\u016Fzn\u00E9 zdroje bun\u011Bk, ale st\u00E1le maj\u00ED hodn\u011B spole\u010Dn\u00E9ho. St\u00E1rnut\u00ED jenyn\u00ED ch\u00E1p\u00E1no jako kombinace mnoha r\u016Fzn\u00FDch proces\u016F, je\u017E ur\u010Duj\u00ED d\u00E9lku \u017Eivota a zdrav\u00ED. Neexistuje jednotn\u00E1 teorie pro v\u0161echny zm\u011Bny k nim\u017E doch\u00E1z\u00ED v pr\u016Fb\u011Bhu st\u00E1rnut\u00ED. Jsme p\u0159esv\u011Bd\u010Deni, \u017Ee je to proces ovlivn\u011Bn\u00FD mnoha faktory. Zam\u011B\u0159\u00EDme se na studium projev\u016Fst\u00E1rnut\u00ED u soubor\u016F mlad\u00FDch a star\u00FDch bun\u011Bk obou model\u016F a na zapojen\u00ED RAS/cAMP dr\u00E1hy a souvisej\u00EDc\u00EDch gen\u016F u kvasinek. K tomu pou\u017Eijeme techniky molekul\u00E1rn\u00ED a bun\u011B\u010Dn\u00E9 biologie, v\u010Detn\u011B cytologick\u00FDch a biochemick\u00FDch analys s d\u016Frazem na ROS a elektronovoumikroskopii. P\u0159edkl\u00E1dan\u00FD projekt p\u0159edstavuje nov\u00FD p\u0159\u00EDstup k pozn\u00E1n\u00ED proces\u016F st\u00E1rnut\u00ED na molekul\u00E1rn\u00ED a bun\u011B\u010Dn\u00E9 \u00FArovni za pou\u017Eit\u00ED bun\u011Bk kvasinky Saccharomyces cerevisiae a o\u010Dn\u00EDch \u010Do\u010Dek. Tento projekt pat\u0159\u00ED k z\u00E1kladn\u00EDmu v\u00FDzkumu, nicm\u00E9n\u011B m\u016F\u017Ee vy\u00FAstit v" . . . . "\u00DAloha genomu a kysl\u00EDkov\u00FDch radik\u00E1l\u016F v procesu st\u00E1rnut\u00ED" . . . "GA301/03/0289" . . . "1"^^ . "Neuvedeno."@en . "1"^^ . . "V\u00A0tomto projektu jsme pou\u017Eili bu\u0148ky kvasinek a sav\u010D\u00EDch o\u010Dn\u00EDch \u010Do\u010Dek jako srovn\u00E1vac\u00ED modely pro studium st\u00E1rnut\u00ED. Tyto bu\u0148ky r\u016Fzn\u00E9ho p\u016Fvodu, ale s mnoh\u00FDmi podobnostmi, n\u00E1m napomohly vid\u011Bt st\u00E1rnut\u00ED v\u00A0rovin\u011B konservativn\u00EDch mechanism\u016F. K\u00A0nim pat\u0159\u00ED vliv bun\u011B"@cs . "http://www.isvav.cz/projectDetail.do?rowId=GA301/03/0289"^^ . . . . . "In this project we intend to use yeast and mammalian ocular lens as model systems for aging studies. These two model systems used in parallel are suitable for comparative aging studies, although they represent two different sources of cells they stillhold many similarities. Aging is today viewed as a combination of many processes, that determine the life span and health. No single theory can account for all the changes that take place in aging. We are convinced that the aging process ismultifactorial. We will focus on the manifestation of the aging processes in the cohorts of young and old cells of both model systems, yeast and ocular lens, and on the involvement of the RAS/cAMP pathway and interacting genes in the aging of the yeastmodel system. For our studies we will use the techniques of molecular and cellular biology, including molecular genetics, cytological and biochemical analyses with the emphasis on reactive oxygen species and electron microscopy. The proposed project"@en . . "2009-01-15+01:00"^^ . . "This project made use of yeast and cells of mammalian ocular lens as comparative model systems for aging studies. These two models of cells from different sources, which exhibit many similarities, helped us to see aging in terms of the conservative mecha"@en .