. "2009-01-01+01:00"^^ . . . "Results describe increasing levels of cytokinins in heat stress and stimulation of their signaling pathways. For plants with elevated biosynhesis or inhibited degradation of cytokinins, main proteins affected by cytokinins including those with anti-oxidation properties were identified. The project objectives were fullfiled."@en . "The role of cytokinins and polyamines in heat stress response and thermotolerance in tobacco and Arabidopsis plants"@en . "GA206/09/2062" . . . "Understanding of mechanisms of heat stress response and thermotolerance in plants has acquired, due to global warming, enhanced significance. Transduction of environmental signals in plants involves phytohormone actions. The role of cytokinins (CK) and polyamines during heat stress and recovery will be evaluated using tobacco and Arabidopsis plants with modulated CK content (by over-expression of genes for CK metabolic enzymes).\u00A0 The impact of heat stress will be followed in roots and leaves at the level of selected transcripts (mitochondrial and nuclear genes for heat shock proteins \u2013 by real time RT PCR), protein spectra (from leaf and root tissues - 2D-electrophoresis) and hormone content (CKs, abscisic acid, salicylic acid, ethylene precursor and auxin \u2013 GC or LC/MS). Polyamine metabolism, expression and activities of selected antioxidant enzymes and content of pigments will contribute to the characterization of the stress impact."@en . . "11"^^ . "2013-06-07+02:00"^^ . . "11"^^ . "Funkce cytokinin\u016F a polyamin\u016F p\u0159i odezv\u011B na teplotn\u00ED stres a p\u0159i zvy\u0161ov\u00E1n\u00ED termotolerance rostlin tab\u00E1ku a Arabidopsis" . "1"^^ . "2014-07-01+02:00"^^ . . . . "V\u00FDsledky projektu popisuj\u00ED zv\u00FD\u0161en\u00ED hladiny cytokinin\u016F p\u0159i teplotn\u00EDm stresu a stimulaci jejich sign\u00E1ln\u00ED dr\u00E1hy. U geneticky upraven\u00FDch rostlin se zv\u00FD\u0161enou biosynt\u00E9zou cytokinin\u016F nebo tam, kde byl aplikov\u00E1n inhibitor jejich degradace, se poda\u0159ilo identifikovat hlavn\u00ED proteiny ovlivn\u011Bn\u00E9 cytokininy v\u010Detn\u011B protein\u016F s antioxida\u010Dn\u00EDm p\u016Fsoben\u00EDm. C\u00EDle projektu byly spln\u011Bny."@cs . "1"^^ . "heat stress cytokinins abscisic acid polyamines Nicotiana tabacum Arabidopsis thaliana"@en . . "http://www.isvav.cz/projectDetail.do?rowId=GA206/09/2062"^^ . . . . . . "2013-12-31+01:00"^^ . "0"^^ . . . . . "Objasn\u011Bn\u00ED mechanism\u016F, kter\u00E9 se pod\u00EDlej\u00ED na odezv\u011B rostlin na teplotn\u00ED stres a jejich toleranci v\u016F\u010Di zv\u00FD\u0161en\u00E9 teplot\u011B, nab\u00FDv\u00E1 vzhledem ke glob\u00E1ln\u00EDmu oteplov\u00E1n\u00ED na praktick\u00E9m v\u00FDznamu. Na p\u0159enosu sign\u00E1l\u016F z vn\u011Bj\u0161\u00EDho prost\u0159ed\u00ED se v rostlin\u00E1ch v\u00FDznamn\u011B pod\u00EDl\u00ED fytohormony. \u00DAloha cytokinin\u016F (CK) a polyamin\u016F (PA) b\u011Bhem teplotn\u00EDho stresu a p\u0159i n\u00E1sledn\u00E9 obnov\u011B bude sledov\u00E1na v rostlin\u00E1ch tab\u00E1ku a Arabidopsis se zv\u00FD\u0161en\u00FDm obsahem CK (v d\u016Fsledku zv\u00FD\u0161en\u00E9 exprese gen\u016F k\u00F3duj\u00EDc\u00EDch enzymy metabolismu CK).\u00A0 Vliv\u00A0 teplotn\u00EDho stresu na listy a ko\u0159eny bude studov\u00E1n na \u00FArovni vybran\u00FDch transkript\u016F (mitochondri\u00E1ln\u00EDch a jadern\u00FDch gen\u016F pro heat-shock proteiny \u2013 pomoc\u00ED real time RT PCR), proteinov\u00FDch spekter (z tk\u00E1n\u00ED list\u016F a ko\u0159en\u016F - 2D-elektrofor\u00E9za) a hladin hormon\u016F (CK, kyselina abscisov\u00E1, kyselina salicylov\u00E1, p\u0159\u00EDm\u00FD prekursor etyl\u00E9nu a auxin \u2013 GC nebo LC/MS). Sledov\u00E1n\u00ED metabolismu PA, exprese a aktivity vybran\u00FDch antioxida\u010Dn\u00EDch enzym\u016F a obsahu \u00A0pigment\u016F p\u0159isp\u011Bje k\u00A0charakterizaci s\u00EDly stresu." .