. . . . . "GA206/02/0967" . . . . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA206/02/0967"^^ . "Plant hormones, which are involved in activation of cyclin-dependent kinases and exhibit cytokinin activity, function as specific triggers of cell cycle progression. Transient accumulation of endogenous cytokinins has been reported at certain stages of cell cycle. This indicates the importance of regulation of cytokinin levels and its role in control of cell division, which is essential for plant development. In contrast to so far frequently used up-regulation of cytokinins by their exogenous application or expression of cytokinin biosynthetic genes of bacterial origin in transformed plant cells, we propose here to explore modulated down-regulation of cytokinins by control of expression and targeting of cytokinin oxidase (CKX3). CKX3 will be produced in Arabidopsis and tobacco plants using Arabidopsis ckx3 gene containing or lacking functional target domain targeting the protein towards secretion and coupled to constitutive or inducible promoters and green fluorescence protein (gfp) coding"@en . . . "0"^^ . . . . . "Cytokininoxid\u00E1za/redukt\u00E1za (CKX) je zapojena do regulace hladin rostlinn\u00FDch hormon\u016F cytokinin\u016F, kter\u00E9 spole\u010Dn\u011B s\u00A0auxinem maj\u00ED kl\u00ED\u010Dovou \u00FAlohu p\u0159i \u0159\u00EDzen\u00ED v\u00FDvoje rostlin. Ned\u00E1vno identifikovan\u00ED \u010Dlenov\u00E9 rodiny CKX se vyzna\u010Duj\u00ED mj. r\u016Fzn\u00FDm c\u00EDlen\u00EDm do r\u016Fzn\u00FDch b"@cs . "1"^^ . "Neuvedeno."@en . . . . "Rostlinn\u00E9 hormony typu cytokinin\u016F, kter\u00E9 aktivuj\u00ED specifick\u00E9 cyklin-z\u00E1visl\u00E9 kin\u00E1zy, p\u016Fsob\u00ED jako specifick\u00E9 aktiv\u00E1tory bun\u011B\u010Dn\u00E9ho cyklu. Kr\u00E1tkodob\u00E1 p\u0159echodn\u00E1 akumulace cytokinin\u016F, kter\u00E1 byla zji\u0161t\u011Bna v ur\u010Dit\u00FDch (kontroln\u00EDch) f\u00E1z\u00EDch bun\u011B\u010Dn\u00E9ho cyklu, nazna\u010Duje v\u00FDznam regulace hladin cytokin\u016F pro kontrolu bun\u011B\u010Dn\u00E9ho d\u011Blen\u00ED a na n\u011Bm z\u00E1visl\u00E9ho v\u00FDvoje rostlin. Na rozd\u00EDl od zvy\u0161ov\u00E1n\u00ED hladin cytokinin\u016F pomoc\u00ED jejich exogenn\u00ED aplikace nebo expres\u00ED p\u0159\u00EDslu\u0161n\u00FDch bakteri\u00E1ln\u00EDch gen\u016F k\u00F3duj\u00EDc\u00EDch enzymy zapojen\u00FDch do jejich biosynt\u00E9zy v\u00A0transgenn\u00EDch rostlin\u00E1ch, p\u0159edkl\u00E1dan\u00FD projekt je zam\u011B\u0159en na ovliv\u0148ov\u00E1n\u00ED hladin cytokinin\u016F modulac\u00ED exprese a c\u00EDlen\u00ED rostlin\u00E9 cytokininoxid\u00E1zy (CKX3). Rostliny Arabidopsis a tab\u00E1ku budou transformov\u00E1ny ckx3 genem s p\u0159irozenou nebo modifikovanou c\u00EDlovou dom\u00E9nou sm\u011Bruj\u00EDc\u00ED enzym k\u00A0sekreci f\u00FAzovan\u00E9ho se sekvenc\u00ED report\u00E9rov\u00E9ho genu gfp pod kontrolou kostitutivn\u00EDho nebo indukovateln\u00E9ho promotoru. Bude stanoven vliv exprese a c\u00EDlen\u00ED CKX3 na biochemick\u00E9 vlastnosti vnitrobun\u011B\u010Dn\u00E9 a" . "2008-06-02+02:00"^^ . . "Targeting of cytokinin oxidase and its role in regulation of plant development"@en . "7"^^ . "7"^^ . . . "V\u00FDznam c\u00EDlen\u00ED cytokininoxid\u00E1zy p\u0159i regulaci v\u00FDvoje rostlin" . ".Cytokinin oxidase/dehydrogenase (CKX) down-regulates the levels of plant hormones cytokinins, which together with auxin play a key role in control of plant development. Members of AtCKX family exhibit different enzyme targeting to different cellular str"@en .