"Dal\u0161\u00ED osud oxida\u010Dn\u011B po\u0161kozen\u00FDch membr\u00E1nov\u00FDch b\u00EDlkovin \u017Eivotn\u011B d\u016Fle\u017Eit\u00FDch pro eukaryontn\u00ED bu\u0148ku: H+-ATPasa plasmatick\u00E9 membr\u00E1ny kvasinek" . . . "13"^^ . . "Reactive oxygen species (ROS) are crucial in the development of a number of diseases, in ageing, programmed cell death and apoptosis. No respiring cell escapes ROS damage since 2-3 % respired O2 is converted to ROS. Plasma membrane is the first target ofROS produced in the environment by pollutants, UV-radiation, ozone and other toxics. In the project we will (1) determine how the oxidative damage of vitally important membrane proteins affects the life function of a cell, (2) define the types of damage or oxidative modifications of the attacked membrane proteins, (3) establish the further fate of oxidatively modified membrane proteins, and (4) determine the ways in which the proteolytic apparatus of the cell participates in elimination of these damageproteins. H2O2 and the Fenton reagent (Fe2+/H2O2) will be used as oxidants and yeast plasma membrane H+-ATPase as model of oxidatively damaged membrane protein. Yeasts are suitable model cells as they exhibit replicative ageing (a simpler analogy of org"@en . "Reaktivn\u00ED druhy kysl\u00EDku (ROS) jsou z\u00E1va\u017En\u00FDm faktorem pod\u00EDlej\u00EDc\u00EDcm se na vzniku mnoha chorob, na st\u00E1rnut\u00ED, programovan\u00E9 smrti bu\u0148ky a apopt\u00F3ze. \u017D\u00E1dn\u00E1 d\u00FDchaj\u00EDc\u00ED bu\u0148ka neunikne jejich \u00FA\u010Dinku, proto\u017Ee 2-3 % respirovan\u00E9ho O2 se p\u0159em\u011Bn\u00ED na ROS. Plasmatick\u00E1 membr\u00E1na je prvn\u00EDm c\u00EDlem ROS vznikaj\u00EDc\u00EDch v okoln\u00EDm prost\u0159ed\u00ED v d\u016Fsledku p\u0159\u00EDtomnosti zn\u011B\u010Di\u0161\u0165uj\u00EDc\u00EDch l\u00E1tek, UV z\u00E1\u0159en\u00ED, ozonu a dal\u0161\u00EDch toxick\u00FDch l\u00E1tek. V projektu (1) stanov\u00EDme, jak oxidativn\u00ED po\u0161kozen\u00ED \u017Eivotn\u011B d\u016Fle\u017Eit\u00FDch membr\u00E1nov\u00FDch b\u00EDlkovin ovlivn\u00ED \u017Eivotn\u00ED funkce bu\u0148ky, (2) budeme definovat typy po\u0161kozen\u00ED nebo oxidativn\u00EDch zm\u011Bn posti\u017Een\u00FDch membr\u00E1nov\u00FDch b\u00EDlkovin, (3) stanov\u00EDme dal\u0161\u00ED osud oxidativn\u011B zm\u011Bn\u011Bn\u00FDch membr\u00E1nov\u00FDch b\u00EDlkovin a (4) stanov\u00EDme zp\u016Fsoby, jimi\u017E se proteolytick\u00FD apar\u00E1t bu\u0148ky pod\u00EDl\u00ED na odstra\u0148ov\u00E1n\u00ED t\u011Bchto po\u0161kozen\u00FDch b\u00EDlkovin. H2O2 a Fentonovo \u010Dinidlo (Fe2+/H2O2) budou pou\u017Eity jako oxidanty a H+-ATPasa plasmatick\u00E9 membr\u00E1ny kvasinek jako model of oxidativn\u011B po\u0161kozen\u00E9 membr\u00E1nov\u00E9 b\u00EDlkoviny. Kvasinky jsou v\u00FDhodn\u00FDm modelem eukaryontn\u00ED bu" . "13"^^ . "Neuvedeno."@en . "Projekt byl zam\u011B\u0159en na studium oxidativn\u00EDho stresu membr\u00E1nov\u00E9 H+ATP\u00E1zy u kvasinek. V\u00FDsledky p\u0159inesly podrobn\u00E9 informace o oxidativn\u00EDch zm\u011Bn\u00E1ch v molekule tohoto enzymu, jeho dal\u0161\u00ED degradaci a vlivu inaktivace ATP\u00E1zy na vitalitu bun\u011Bk. Pr\u00E1ce rovn\u011B\u017E p\u0159ines"@cs . "2003-04-01+02:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GA204/99/0488"^^ . "GA204/99/0488" . "0"^^ . . . . "Oxidatively damaged vital eukaryotic membrane proteins and their further fate: yeast plasma membrane H+-ATPase"@en . . . "0"^^ . . . . "1"^^ . . . . . .