"8"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA204/99/0367"^^ . "8"^^ . . "V n\u00E1vaznosti na projekty rozpracovan\u00E9 v laborato\u0159i navrhovatele budou zkoum\u00E1ny molekul\u00E1rn\u00ED detaily \u010Dasn\u00FDch f\u00E1z\u00ED signaliza\u010Dn\u00EDch mechanism\u016F pou\u017E\u00EDvan\u00FDch imunoreceptory (antigenn\u011B specifick\u00E9 receptory T a B lymfocyt\u016F (TCR, BCR) a Fc-receptory). Hlavn\u00EDm c\u00EDlembude (1) zjistit pomoc\u00ED nov\u00FDch elektroforetick\u00FDch metod, jak\u00E1 je %22nativn\u00ED%22 supramolekul\u00E1rn\u00ED struktura t\u011Bchto receptorov\u00FDch komplex\u016F; (2) potvrdit hypot\u00E9zu, \u017Ee prvn\u00EDm krokem zahajuj\u00EDc\u00EDm p\u0159enos sign\u00E1lu po ligaci imunoreceptoru je agregace n\u00E1sledovan\u00E1 bezprost\u0159edn\u011B p\u0159ipojen\u00EDm k membr\u00E1nov\u00FDm mikrodom\u00E9n\u00E1m bohat\u00FDm na Src-kin\u00E1zy a jin\u00E9 signaliza\u010Dn\u00ED molekuly; (3) objasnit slo\u017Een\u00ED endocytick\u00FDch v\u00E1\u010Dk\u016F, kter\u00E9 se tvo\u0159\u00ED po ligaci a agregaci imunoreceptor\u016F. Projekt bude \u0159e\u0161en pomoc\u00ED biochemick\u00FDch, molekul\u00E1rn\u011B biologick\u00FDch a imunochemick\u00FDch metod a jeho v\u00FDsledky p\u0159isp\u011Bj\u00ED k objasn\u011Bn\u00ED kritick\u00FDch \u00FAvodn\u00EDch f\u00E1z\u00ED aktivace bun\u011Bk imunitn\u00EDho syst\u00E9mu." . . "1"^^ . . . . . . "Molecular mechanisms of signalling through immunoreceptors"@en . . "0"^^ . . "Molekul\u00E1rn\u00ED mechanismy signalizace imunoreceptory" . "GA204/99/0367" . "0"^^ . . "Grant byl p\u0159ed\u010Dasn\u011B ukon\u010Den z d\u016Fvodu ud\u011Blen\u00ED N\u00E1rodn\u00EDho v\u00FDzkumn\u00E9ho centra. Projekt ukon\u010Den o rok d\u0159\u00EDve proto, aby nedo\u0161lo k p\u0159ekryvu s projektem M\u0160MT. Bylo dosa\u017Eeno v\u011Bt\u0161iny pl\u00E1novan\u00FDch c\u00EDl\u016F, dosud nebyla dokon\u010Dena pl\u00E1novan\u00E1 \u010D\u00E1st, t\u00FDkaj\u00EDc\u00ED se stechiometrie"@cs . . "Based on previous projects of our laboratory, molecular details of early phases of signalling processes used by immunoreceptors (TCR, BCR, Fc-receptors) will be examined. The main aims will be (1) To determine by means of novel electrophoretic methods the %22native%22 supramolecular structure of these receptor complexes; (2) To confirm the hypothesis that the first step initiating signal transduction after the immunoreceptor ligation is aggregation followed immediately by attachment to membrane microdomains rich in Src/kinases and other signalling molecules; (3) To elucidate molecular composition of the endocytic vesicles formed after ligation and aggregation of the immunoreceptors. The project will be solved using biochemical, molecular biological and immunochemical methods and the results will contribute to better understanding of the critical very early phases of activation of the immune system's cells."@en . . . . . .