. . "GA204/98/0043" . . "1"^^ . "Structural and functional analysis of circadian clock genes"@en . . "0"^^ . "Strukturn\u00ED a funk\u010Dn\u00ED anal\u00FDza gen\u016F cirkadi\u00E1nn\u00EDch biologick\u00FDch hodin" . "1"^^ . . "1"^^ . "Two circadian clock genes, period (per) and timeless (tim), have been studied intensively in Drosophila. Rr\u00EBcently, we demonstrated the presence of a functional homolog of per in the silkmoth Antheraea pernyi and cloned a homologous fragment of per from the cockroach Periplaneta americana. The presence of these homologs indicates for the first time that the molecular mechanisms that underlie circadian clock may be evolutionary conserved among insects, and potentionally beyond this phylogenetic class. Wenow propose to extend what is known about the molecular basis of circadian clocks in these two nondipteran species. The specific aims are to 1) determine the location of circadian clock cells in silkmoth brain by evaluating whether 8 PER-producing neurosecretory cells are actual clock cells and cloning and characterizing silkmoth tim; and 2) demonstrate that the cockroach per homolog is a functional clock homolog by defining its molecular oscillations and delineating whether cockroach per can func"@en . . "Molekul\u00E1rn\u00ED regulace gen\u016F cirkadi\u00E1nn\u00EDch biologick\u00FDch hodin period a timeless u martin\u00E1\u010De a \u0161v\u00E1ba jsou zcela odli\u0161n\u00E9 od Drosophily. V obou p\u0159\u00EDpadech nedoch\u00E1z\u00ED k translokaci produkt\u016F cirkadi\u00E1nn\u00EDch gen\u016F do j\u00E1dra. PER-pozitivn\u00ED bu\u0148ky u obou druh\u016F exprimuj\u00ED a"@cs . . . . . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA204/98/0043"^^ . "U Drosophila melanogaster byly intenzivn\u011B studov\u00E1ny dva cirkadi\u00E1nn\u00ED geny, period (per) a timeless (tim). Ned\u00E1vno se n\u00E1m poda\u0159ilo prok\u00E1zat p\u0159\u00EDtomnost funk\u010Dn\u00EDho homologu per u martin\u00E1\u010De Antheraea pernyi a tak\u00E9 zaklonovat homologick\u00FD fragment genu per ze \u0161v\u00E1ba Periplaneta americana. Existence t\u011Bchto homolog\u016F poprv\u00E9 nazna\u010Duje, \u017Ee molekul\u00E1rn\u00ED mechanismy tvo\u0159\u00EDc\u00ED podstatu cirkadi\u00E1nn\u00EDch hodin mohou b\u00FDt u hmyzu evolu\u010Dn\u011B konzervov\u00E1ny. Navrhujeme nyn\u00ED roz\u0161\u00ED\u0159it co je zn\u00E1mo o molekul\u00E1rn\u00EDch mechanismech cirkadi\u00E1nn\u00EDch hodin u t\u011Bchto dvou hmyz\u00EDch druh\u016F. Specifick\u00E9 c\u00EDle jsou 1) ur\u010Dit m\u00EDsto cirkadi\u00E1nn\u00EDch bun\u011Bk v mozku martin\u00E1\u010De, a 2) prok\u00E1zat, \u017Ee homolog genu per u \u0161v\u00E1ba je funk\u010Dn\u00ED homolog cirkadi\u00E1nn\u00EDho genu. Dlouhodob\u00FDm c\u00EDlem tohoto v\u00FDzkumn\u00E9ho pl\u00E1nu je z\u00EDskat lep\u0161\u00ED porozum\u011Bn\u00ED bun\u011B\u010Dn\u00FDch a molekul\u00E1rn\u00EDch mechanism\u016F, kter\u00E9 tvo\u0159\u00ED podstatu cirkadi\u00E1nn\u00ED rytmicity. Cirkadi\u00E1nn\u00ED oscilace ovliv\u0148uj\u00ED doslova v\u0161echny aspekty lidsk\u00E9 fyziologie a mohou v\u00FDznamn\u011B p\u0159isp\u00EDvat k patofyziologick\u00FDm stav\u016Fm. D\u016Fkladn\u011Bj\u0161\u00ED porozum\u011B" . . . . .