"Kvasinkov\u00E1 bu\u0148ka sleduje p\u0159i sv\u00E9mvegetativn\u00EDm v\u00FDvoji geneticky definovan\u00FD program, kter\u00FD obsahuje vysoce konzervativn\u00ED prvky spole\u010Dn\u00E9 s dal\u0161\u00EDmi eukaryontn\u00EDmi syst\u00E9my. V t\u00E9to oblasti navrhujeme nad\u00E1le rozv\u00EDjet kvasinky jako modelov\u00E9 organismy pro identifikaci nov\u00FDch protein\u016F interaguj\u00EDc\u00EDch s mikrotubuly, se zvl\u00E1\u0161tn\u00EDm z\u0159etelem na jejich potenci\u00E1ln\u00ED funkci p\u0159i ur\u010Dov\u00E1n\u00ED polohy d\u011Bl\u00EDc\u00EDho v\u0159et\u00E9nka a p\u0159i reorganisaci cytoskeletu vlivem vn\u011Bj\u0161\u00EDch (r\u016Fstov\u00FDch) sign\u00E1l\u016F. Pro vyhled\u00E1v\u00E1n\u00ED interaguj\u00EDc\u00EDch protein\u016F a odpov\u00EDdaj\u00EDc\u00EDch gen\u016F budou vyu\u017Eity nov\u00E9 heterologn\u00ED protil\u00E1tky. Prostorov\u00E9 vztahy mezi interaguj\u00EDc\u00EDmi proteiny a mikrotubuly na \u00FArovni bu\u0148ky budou sledov\u00E1ny konfok\u00E1ln\u00ED laserovou mikroskopi\u00ED. Vz\u00E1jemn\u00E9 interakce budou d\u00E1le analyzov\u00E1ny pomoc\u00ED cdc, cytoskelet\u00E1ln\u00EDch i nov\u011B zkonstruovan\u00FDch podm\u00EDn\u011Bn\u00FDch mutant\u016F. Bude studov\u00E1n vliv fosforylace a defosforylace protein\u016F p\u0159i t\u011Bchto interakc\u00EDch. O\u010Dek\u00E1v\u00E1me, \u017Ee z\u00EDskan\u00E9 poznatky p\u0159isp\u011Bj\u00ED k nov\u00E9mu pohledu na komunikaci j\u00E1dra s bun\u011B\u010Dnou periferi\u00ED prost\u0159ednictv\u00EDm mikrotubul\u016F a" . "0"^^ . . . "1"^^ . . . . . . . . "\u00DAloha cytoplazmatick\u00FDch mikrotubul\u016F a asociovan\u00FDch protein\u016F v morfogenezi kvasinek" . . . . . "15"^^ . "1. Hlavn\u00ED p\u0159\u00EDnos k pozn\u00E1n\u00ED v bun\u011B\u010Dn\u00E9 biologii je d\u00EDl\u010D\u00ED konkretizace vztahu mezi cytoskeletem a translac\u00ED. Byl identifikov\u00E1n gen (Rpg 1) jeho\u017E produkt je podjednotkou transla\u010Dn\u00EDho faktoru 3 (eIF3) a kolokalizuje s mikrotubuly. Zodpov\u00EDd\u00E1 za lokalizovanou p"@cs . . "Vegetative development of yeast cell follows an intimate genetically defined program. In addition, there is a remarkable conservation in components that comprise developmental machinery between yeast and other eukaryotes. This proposal seeks support to continue our development of yeast as a model system to identify new yeast proteins interacting with microtubules to maintain mitotic spindle orientation as well as those involved in a complex interplay with growth signals resulting in polarized cytoskeleton rearrangement. In the search for these proteins and corresponding genes new heterologous antibodies will be applied. Cellular localization of these proteins to microtubules will be analyzed by confocal laser scan microscopy. Various cdc, cytoskeletal and newlyconstructed conditional mutants will be investigated to evaluate the role for these microtubule interactions. The involvement of protein phosphorylation/dephosphorylation in this process will be studied. The information gained should contribute"@en . "15"^^ . "Role of cytoplasmic microtubules and associated proteins in yeast morphogenesis"@en . "GA204/96/1368" . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA204/96/1368"^^ .