. "This project aims at bacterial transporters of divalent metal ions (DMI) from the Nramp/MntH family counting among the proton-dependent secondary active membrane transport proteins. Although the Nramp-related proteins, playing a key role in various physiological processes including macrophage-pathogen interaction, are present in virtually all organisms, the transport mechanism is not understood. We propose to advance knowledge of the structure-function relationship in the MntH/Nramp family by studying the function of key residues in vivo, in vitro (right-side out vesicles, in-side out vesicles and proteoliposomes) and in a peptide-membrane model system. The peptide model allows complementing the functional assays by information about transmembrane segment structure, insertion and folding, and possibly some functional details. We expect to collect data leading to the elucidation of DMI- and proton- translocation pathway in the MntH and to the characterization of some aspects of"@en . . "Projekt je zam\u011B\u0159en na studium bakteri\u00E1ln\u00EDch transport\u00E9r\u016F dvojmocn\u00FDch kovov\u00FDch iont\u016F z rodiny Nramp/MntH pat\u0159\u00EDc\u00EDch mezi sekund\u00E1rn\u011B aktivn\u00ED membr\u00E1nov\u00E9 transportn\u00ED proteiny z\u00E1visl\u00E9 na protonov\u00E9m gradientu. P\u0159esto\u017Ee jsou tyto proteiny, kter\u00E9 hraj\u00ED kl\u00ED\u010Dovou roli v r\u016Fzn\u00FDch fyziologick\u00FDch procesech v\u010Detn\u011B interakce makrof\u00E1g-patogen, p\u0159\u00EDtomn\u00E9 prakticky ve v\u0161ech organismech, nen\u00ED mechanismus transportu zn\u00E1m. N\u00E1vrh po\u010D\u00EDt\u00E1 s prohlouben\u00EDm znalost\u00ED o vztahu struktury a funkce prost\u0159ednictv\u00EDm studia vlivu kl\u00ED\u010Dov\u00FDch rezidu\u00ED na funkci membr\u00E1nov\u00FDch transport\u00E9r\u016F MntH in vivo, in vitro (membr\u00E1nov\u00E9 vesikuly a proteoliposomy) a v modelov\u00E9m syst\u00E9mu peptid-membr\u00E1na. Tento model umo\u017E\u0148uje doplnit poznatky z\u00EDskan\u00E9 funk\u010Dn\u00EDmi testy informacemi o struktu\u0159e a sbalen\u00ED transmembr\u00E1nov\u00FDch segment\u016F a eventu\u00E1ln\u011B n\u011Bkter\u00FDmi funk\u010Dn\u00EDmi detaily. O\u010Dek\u00E1v\u00E1 se, \u017Ee z\u00EDskan\u00E1 data povedou k objasn\u011Bn\u00ED transloka\u010Dn\u00ED dr\u00E1hy pro protony a dvojmocn\u00E9 ionty uvnit\u0159 MntH a k charakterizaci n\u011Bkter\u00FDch aspekt\u016F fylogenetick\u00E9 odli\u0161nosti v r\u00E1mci" . . "2015-02-09+01:00"^^ . . . " Nramp" . . . " MntH" . "2010-04-16+02:00"^^ . "1"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA204/07/0558"^^ . "divalent metal ions" . "3"^^ . "0"^^ . "3"^^ . "Projekt byl zam\u011B\u0159en na studium bakteri\u00E1ln\u00EDch transport\u00E9r\u016F dvojmocn\u00FDch kovov\u00FDch iont\u016F z rodiny Nramp/MntH (SLC11) pat\u0159\u00EDc\u00EDch mezi sekund\u00E1rn\u011B aktivn\u00ED membr\u00E1nov\u00E9 transportn\u00ED proteiny z\u00E1visl\u00E9 na protonov\u00E9m gradientu. Projekt p\u0159isp\u011Bl k objasn\u011Bn\u00ED vztahu struktury a funkce v rodin\u011B Nramp pomoc\u00ED studia funkce kl\u00ED\u010Dov\u00FDch rezidu\u00ED/transmembr\u00E1nov\u00FDch segment\u016F, in vivo, in vitro a v modelov\u00E9m syst"@cs . . "0"^^ . "Biophysical study of MntH membrane transport proteins: structure-function relationship"@en . . . "2010-12-31+01:00"^^ . "Biofyzik\u00E1ln\u00ED studie membr\u00E1nov\u00FDch transportn\u00EDch protein\u016F MntH: vztah struktury a funkce" . "divalent metal ions; membrane transport; MntH; Nramp; secondary active membrane transport proteins"@en . . . . . . . . "This project aimed at bacterial H+-dependent secondary active membrane transporters of divalent metal ions (Me2+) from the Nramp/MntH (SLC11) family. It advanced knowledge of the structure-function relationship in the MntH/Nramp family by studying the function of key residues in vivo, in vitro and in a peptide-membrane model system. A new fluorescence method developed for measuring the stoichiome"@en . . "2007-01-01+01:00"^^ . . . " membrane transport" . "GA204/07/0558" .