"2008-12-31+01:00"^^ . "Trypanosoma brucei and related kinetoplastid flagellates are early-branching protozoans responsible for serious diseases of man and animals. Their single cell, and in particular their mitochondrion, contain many unique features. We propose to perform, using RNA interference, functional analysis of proteins that participate in iron sulfur cluster assembly, according to recent views one of the most essential pathways in the organelle. By down-regulating putative subunits of respiratory complex I\u00A0we willaddress the question whether this complex is absent or present in the T. brucei procyclic form. Moreover, we will develop or modify for their application in T. brucei a number of tests for various functions of the trypanosomatid organelle. These tests will be very useful for functional analyses of mitochondrial proteins identified in frame of the recently completed genome project."@en . . . . . " mitochondrion" . . . . . . . "Functional analysis of mitochondrial proteins in Trypanosoma brucei"@en . "trypanosoma; mitochondrion; RNAi; Fe-S proteins; respiration"@en . "Funk\u010Dn\u00ED anal\u00FDza mitochondri\u00E1ln\u00EDch protein\u016F u Trypanosoma brucei" . "GA204/06/1558" . . " Fe-S proteins" . "14"^^ . . "14"^^ . "trypanosoma" . "http://www.isvav.cz/projectDetail.do?rowId=GA204/06/1558"^^ . "2006-01-01+01:00"^^ . "0"^^ . "2009-10-22+02:00"^^ . " RNAi" . "0"^^ . . "Trypanosoma brucei a p\u0159\u00EDbuzn\u00ED bi\u010D\u00EDkovci \u0159adu Kinetoplastida pat\u0159\u00ED mezi nej\u010Dasn\u011Bj\u0161\u00ED eukaryotick\u00E9 v\u011Btve a zp\u016Fsobuj\u00ED velmi z\u00E1va\u017En\u00E1 onemocn\u011Bn\u00ED \u010Dlov\u011Bka a zv\u00ED\u0159at. Tito prvoci se vyzna\u010Duj\u00ED \u0159adou neobvykl\u00FDch vlastnost\u00ED a struktur a zejm\u00E9na jejich mitochondrie jejedine\u010Dnou organelou. Dle sou\u010Dasn\u00FDch p\u0159edstav je jednou z\u00A0nejz\u00E1kladn\u011Bj\u0161\u00EDch funkc\u00ED mitochondrie tvorba komplex\u016F s\u00EDry a \u017Eeleza. C\u00EDlem p\u0159edkl\u00E1dan\u00E9 \u017E\u00E1dosti je funk\u010Dn\u00ED anal\u00FDza protein\u016F \u00FA\u010Dastn\u00EDc\u00EDch se t\u00E9to dr\u00E1hy u T. brucei. D\u00E1le budeme metodami interference RNA studovat i funkci mo\u017En\u00FDch podjednotek respira\u010Dn\u00EDho komplexu I a vyj\u00E1d\u0159\u00EDme se k\u00A0\u017Eiv\u011B diskutovan\u00E9 ot\u00E1zce existence tohoto komplexu u procyklick\u00FDch st\u00E1di\u00ED parazita. V\u00A0r\u00E1mci funk\u010Dn\u00EDch anal\u00FDz t\u011Bchto dvou skupin protein\u016F zavedeme (\u010Di budeme modifikovat propou\u017Eit\u00ED u trypanosom) sadu test\u016F umo\u017E\u0148uj\u00EDc\u00EDch analyzovat funkce mitochondri\u00E1ln\u00EDch protein\u016F identifikovan\u00FDch v\u00A0r\u00E1mci pr\u00E1v\u011B zcela sekvenovan\u00E9ho genomu." . "Popsali jsme funkce n\u011Bkolika pro \u017Eivot nezbytn\u00FDch mitochondri\u00E1ln\u00EDch protein\u016F u Trypanosoma brucei, kter\u00E9 se z\u00FA\u010Dast\u0148uj\u00ED synt\u00E9zy \u017Eelezosirn\u00FDch klaster\u016F a editov\u00E1n\u00ED RNA. Nejd\u016Fle\u017Eit\u011Bj\u0161\u00ED je zji\u0161t\u011Bn\u00ED, \u017Ee p\u0159i vypnut\u00ED trypanosomov\u00E9ho frataxinu v bu\u0148ce trypanosom"@cs . "1"^^ . . "We have established the functions of several essential mitochondrial proteins of Trypanosoma brucei, which participate in iron sulfur cluster synthesis and RNA editing. The most important finding is that human frataxin can rescue the disruption of trypan"@en . . . "2008-04-25+02:00"^^ . . .