. "2008-04-25+02:00"^^ . "chromatin" . . . . . " oocytes" . "2008-12-31+01:00"^^ . "Byla prok\u00E1z\u00E1na p\u0159\u00EDtomnost kondenzinoveho komplexu II (CAP-C, CAP-E, CAP-D3, CAP-G a CAP-H2) v sav\u010D\u00EDch oocytech. D\u00E1le bylo potvrzeno, \u017Ee v pr\u016Fb\u011Bhu meiotick\u00E9ho zr\u00E1n\u00ED doch\u00E1z\u00ED k n\u00E1r\u016Fstu fosforylace podjednotky CAP-D3, na druh\u00E9 stran\u011B nedoch\u00E1z\u00ED k sumoylaci \u017E\u00E1"@cs . . . . . . "chromatin; meiotic maturation; oocytes; phosphorylation; chromosomal aneuploidy"@en . "http://www.isvav.cz/projectDetail.do?rowId=GA204/06/1297"^^ . . . "V p\u0159edkl\u00E1dan\u00E9m projektu navrhujeme sledovat mechanismy pod\u00EDlej\u00EDc\u00ED se na regulaci zm\u011Bn ve struktu\u0159e a konformaci chromatinu b\u011Bhem meiotick\u00E9ho zr\u00E1n\u00ED prase\u010D\u00EDch oocyt\u016F; zam\u011B\u0159\u00EDme se p\u0159edev\u0161\u00EDm na \u00FAlohu postransla\u010Dn\u00EDch modifikac\u00ED jednotliv\u00FDch podjednotek 13S kondenzinov\u00E9ho komplexu a \u00FAlohu Aurora B kin\u00E1zy pro kondenzaci a segregaci chromozom\u016F, stejn\u011B tak jako na \u00FAlohu fosforylace obecn\u011B a konkr\u00E9tn\u011B i na \u00FAlohu fosforylace histonu H3 p\u0159i kondenzaci chromatinu. Prase\u010D\u00ED oocyty jsou dobr\u00FDm modelem pro sledov\u00E1n\u00ED zm\u011Bn jednotliv\u00FDch enzymatick\u00FDch aktivit, zejm\u00E9na vzhledem k relativn\u011B dlouh\u00E9 dob\u011B meiotick\u00E9ho zr\u00E1n\u00ED (44 hodin) a vzhledem k\u00A0vysok\u00E9 synchronizaci oocyt\u016F v jednotliv\u00FDch st\u00E1di\u00EDch zr\u00E1n\u00ED. V\u00FDsledky z\u00EDskan\u00E9 na vzorc\u00EDch z\u00A0jate\u010Dn\u00EDho materi\u00E1lu pak budou vyu\u017Eity pro studium mo\u017En\u00E9ho v\u00FDskytu aneuploidie a dal\u0161\u00EDch chromozom\u00E1ln\u00EDch abnormalit u prasni\u010Dek z\u00A0na\u0161eho chovu miniaturn\u00EDch prasat v\u00A0z\u00E1vislosti na v\u011Bku samic a detekci jejich mo\u017En\u00FDch p\u0159\u00ED\u010Din." . . "Zm\u011Bny ve struktu\u0159e chromatinu b\u011Bhem meiotick\u00E9ho zr\u00E1n\u00ED prase\u010D\u00EDch oocyt\u016F ve vztahu k chromozom\u00E1ln\u00ED aneuploidii" . "The changes in chromatin structure during meiotic maturation of pig oocytes: correlation to chromosomal aneuploidy"@en . "GA204/06/1297" . "In this project we propose to follow the mechanisms involved in the regulation of the changes in chromatin structure and conformation during meiotic maturation of pig oocytes; we will study the role of posttranslational modification(s) of 13S condensin complex subunits, as well as Aurora B kinase in the process of chromosome condensation and seggregation, the role of overall phosphorylation changes and in particular of histone H3 phosphorylation in chromatin condensation. The oocytes of these species represent a good model for study of changes in different enzymatic activities during meiosis, because of the relatively long period of meiotic division (44 hrs) and because of high synchronization of oocytes in different maturational stages. The results obtained from slaughterhouse samples will be used for the study of possible occurrence of aneuploidy and other chromosomal abnormalities in females from our breed of miniature pigs in correlation with their age and for the detection of the possible"@en . " meiotic maturation" . . " phosphorylation" . . "2006-01-01+01:00"^^ . "The condensin II complex (CAP-C, CAP-E, CAP-D3, CAP-G a CAP-H2) was shown to be present in mammalian oocytes. It was proved that the subunit CAP-D3 became gradually phosphorylated during meiotic maturation, on the other side, sumoylation of condensin sub"@en . "3"^^ . "0"^^ . . "3"^^ . "1"^^ . . . "0"^^ . "2009-10-22+02:00"^^ . .