"The essential gene RPG1/TIF32 of Saccharomyces cerevisiae encodes the large subunit of the translation initiation factor eIF3. We found that besides the interactions with other eIF3 subunits it localizes to microtubules and physically interacts with the actin-associated protein Sla2. In this project we propose to continue the functional analysis of the cytoskeletal interactions of Rpg1p and the eIF3 complex. With the use of modern techniques of the yeast molecular cell biology we will explore a hypothesis that Rpg1 is the eIF3-cytoskeleton bridging protein. The experiments suggested would include a complete analysis of Rpg1p interactions, constructions of intragenously tagged deletion derivatives of Rpg1p and Rpg1p-interacting proteins, constructions of new rpg1 mutants and their phenotypic analysis, co-localizations of Rpg1p with interacting partners and elucidation of the effect of Rpg1p phosphorylation on its distribution. Since the human ortholog of Rpg1p, protein p170, appeared to"@en . . . . . "Neuvedeno."@en . "Role of cytoskeletal interactions of the translation initiation factor eIF3 in yeast morphogenesis"@en . "0"^^ . . "18"^^ . . "18"^^ . "2008-06-02+02:00"^^ . . "GA204/02/1424" . . . "\u00DAloha cytoskelet\u00E1ln\u00EDch interakc\u00ED transla\u010Dn\u00EDho inicia\u010Dn\u00EDho faktoru eIF3 v morfogenezi kvasinek" . "http://www.isvav.cz/projectDetail.do?rowId=GA204/02/1424"^^ . "Esenci\u00E1ln\u00ED gen RPG1/TIF32 kvasinky Saccharomyces cerevisiae k\u00F3duje velkou podjednotku transla\u010Dn\u00EDho inicia\u010Dn\u00EDho faktoru eIF3. Zjistili jsme, \u017Ee krom\u011B interakc\u00ED s jin\u00FDmi podjednotkami eIF3 Rpg1p lokalizuje na mikrotubuly a fyzicky interaguje s aktin-asociovan\u00FDm proteinem Sla2. V tomto projektu navrhujeme pokra\u010Dov\u00E1n\u00ED funk\u010Dn\u00ED anal\u00FDzy cytoskelet\u00E1rn\u00EDch interakci Rpg1p a cel\u00E9ho eIF3 komplexu. S vyu\u017Eit\u00EDm modern\u00EDch technik kvasinkov\u00E9 molekul\u00E1rn\u00ED bun\u011B\u010Dn\u00E9 biologie budeme ov\u011B\u0159ovat hypot\u00E9zu, \u017Ee Rpg1p je proteinem propojuj\u00EDc\u00EDm komplex eIF3 s cytoskeletem. Navrhovan\u00E9 experimenty zahrnuj\u00ED kompletn\u00ED anal\u00FDzu Rpg1p interakc\u00ED, konstrukci intragenov\u011B zna\u010Den\u00FDch dele\u010Dn\u00EDch deriv\u00E1t\u016F Rpg1p a jeho interak\u010Dn\u00EDch partner\u016F, konstrukci nov\u00FDch rpg1 mutant\u016F a jejich fenotypickou anal\u00FDzu, kolokalizaci Rpg1p s interak\u010Dn\u00EDmi partnery a objasn\u011Bn\u00ED vlivu fosforylace na distribuci Rpg1p. Jeliko\u017E lidsk\u00FD ortolog Rpg1p, protein p170, pat\u0159\u00ED mezi v\u00FDznamn\u00E9 markery zhoubn\u00E9ho bujen\u00ED, p\u0159edpokl\u00E1d\u00E1me, \u017Ee tak\u00E9 na\u0161e v\u00FDsledky by m\u011Bly" . . . . "Projekt se t\u00FDkal objasn\u011Bn\u00ED \u00FAlohy cytoskelet\u00E1ln\u00EDch interakc\u00ED transla\u010Dn\u00EDho inicia\u010Dn\u00EDho faktoru eIF3a/Rpg1 v morfogenezi S.cerevisiae. D\u016Fle\u017Eit\u00E9 bylo zejm\u00E9na na\u0161e zji\u0161t\u011Bn\u00ED, \u017Ee morfologick\u00E9 aberace hostitelsk\u00E9ho kmene nadprodukuj\u00EDc\u00EDho S.pombe homologa eIF3a j"@cs . . "The project concerned the function of cytoskeletal interaction of translation initiation factor eIF3a/Rpg1p in S.cerevisiae morphogenesis. We show that morphological aberrations of the host strain overproducing the S.pombe homolog of eIF3a might be a con"@en . . . . . . . "0"^^ . . . "1"^^ .