"Bun\u011B\u010Dn\u00E1 st\u011Bna kvasinek jako extracelul\u00E1rn\u00ED matrix: cytoskelet a st\u011Bnov\u00E9 funkce" . . . "18"^^ . "Neuvedeno."@en . "18"^^ . . . . . . "Z hlediska sv\u00E9ho p\u016Fvodu, chemick\u00E9ho slo\u017Een\u00ED a struktury m\u016F\u017Ee b\u00FDt bun\u011B\u010Dn\u00E1 st\u011Bna pova\u017Eov\u00E1na za extracellul\u00E1rn\u00ED matrix, doklady o jej\u00EDm struktur\u00E1ln\u00EDm a funk\u010Dn\u00EDm spojen\u00ED s cytoplazmou v\u0161ak st\u00E1le chyb\u00ED. C\u00EDlem projektu je objasnit vztahy cytoskelet\u00E1ln\u00EDch komponent - aktinu a mikrotubul\u016F - a bun\u011B\u010Dn\u00E9 st\u011Bny za situac\u00ED, kdy bun\u011B\u010Dn\u00E1 st\u011Bna roste, vytv\u00E1\u0159\u00ED se de novo nebo se zvy\u0161uje jej\u00ED turnover, tj. p\u0159i vegetativn\u00EDm r\u016Fstu bu\u0148ky, p\u0159i sexu\u00E1ln\u00EDm p\u00E1rov\u00E1n\u00ED, p\u0159i sporulaci a v pr\u016Fb\u011Bhu regenerace protoplast\u016F. Budou hled\u00E1ny spojovac\u00ED \u010Dl\u00E1nky mezi cytoplazmou a bun\u011B\u010Dnou st\u011Bnou ve smyslu transmembr\u00E1nov\u00FDch protein\u016F typu integrin\u016F, d\u00E1le molekuly typu linker\u016F mezi cytoskeletem a transmembr\u00E1nov\u00FDmi proteiny a rovn\u011B\u017E interakce mezi t\u011Bmito proteiny a st\u011Bnov\u00FDmi polymery. Jako modely budou pou\u017Eity pu\u010D\u00EDc\u00ED kvasinky Saccharomyces cerevisiae a p\u0159\u00ED\u010Dn\u011B se d\u011Bl\u00EDc\u00ED Schizosaccharomyces pombe za r\u016Fzn\u00FDch f\u00E1z\u00ED \u017Eivotn\u00EDho cyklu a protoplasty t\u011Bchto bun\u011Bk regeneruj\u00EDc\u00ED bun\u011B\u010Dnou st\u011Bnu. D\u00E1le jsou k dispozici mutovan\u00E9 kmeny t\u011Bchto kvasinek s" . . "2008-05-19+02:00"^^ . . "In terms of origin, chemical composition and structure, the cell wall can be regarded as an extracellular matrix, but evidence of its structural and functional relation to the cytoplasm is still missing. The objective of the project is to elucidate relationships between cytoskeletal components (actin and tubulin) and the cell wall during its growth, de novo formation, in the period of increased turnover, i.e., during cell vegetative growth, at mating, at sporulation and during protoplast regeneration. The project will also include investigations into links between the cytoplasm and cell wall by means of transmembrane proteins of integrin type, into molecular linkers between the cytoskeleton and transmembrane proteins and into interactionbetween these proteins and cell wall polymers. The microorganisms used as models will be the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe in various phases of the cell cycle, the protoplasts of these species"@en . "1"^^ . "GA204/00/0394" . "0"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GA204/00/0394"^^ . . . "0"^^ . . . "P\u0159i \u0159e\u0161en\u00ED projektu byly z\u00EDsk\u00E1ny nov\u00E9 \u00FAdaje o vztahu regenerace bun\u011B\u010Dn\u00E9 st\u011Bny kvasinek a regenerace aktinu, o funkci produktu genu cps6 Schizosaccharomyces pombe pro integritu bun\u011B\u010Dn\u00E9 st\u011Bny a o existenci protein\u016F s antigenn\u00EDmi vlastnostmi \u017Eivoti\u010Di\u0161n\u00E9ho s"@cs . . "The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions"@en . . . .