"0"^^ . "2009-01-01+01:00"^^ . . . . "0"^^ . " of" . . "Dle hlavn\u00EDho c\u00EDle projektu, \u0159e\u0161itelsk\u00FD t\u00FDm v\u00FDznamn\u011B p\u0159isp\u011Bl k prohlouben\u00ED znalost\u00ED o RNA, fyzik\u00E1ln\u00ED chemii molekulov\u00FDch interakc\u00ED modul\u00E1rn\u00EDch \u010D\u00E1st\u00ED RNA i o zp\u016Fsobech za\u010Dlen\u011Bn\u00ED RNA do v\u011Bt\u0161\u00EDch celk\u016F.Projekt byl vy\u0159e\u0161en na vynikaj\u00EDc\u00ED \u00FArovni. Dokladem je i 49 publikac\u00ED v impaktovan\u00FDch \u010Dasopisech."@cs . "structure" . " chemistry" . . "Studium z\u00E1kladn\u00EDch princip\u016F, jak molekulov\u00E9 interakce ovliv\u0148uj\u00ED folding a funkci velk\u00FDch molekul RNA, jako je ribosom, s pou\u017E\u00EDt\u00EDm nejmodern\u011Bj\u0161\u00EDch metod po\u010D\u00EDta\u010Dov\u00E9 chemie (po\u010D\u00EDta\u010Dov\u00FDch simulac\u00ED s explicitn\u00EDm zahrnut\u00EDm solventu a kvantov\u00E9 chemie) a bioinformatiky, v \u00FAzk\u00E9 spolupr\u00E1ci s experiment\u00E1ln\u00EDmi laborato\u0159emi. Projekt je zam\u011B\u0159en na energetiku molekulov\u00FDch interakc\u00ED a stochastick\u00E9 pohyby z\u00E1kladn\u00EDch stavebn\u00EDch modul\u016F RNA. Jak energetika, tak stochastick\u00E9 pohyby jsou mimo\u0159\u00E1dn\u011B d\u016Fle\u017Eit\u00E9 vlastnosti, kter\u00E9 ale lze jen velmi omezen\u011B charakterizovat dostupn\u00FDmi experiment\u00E1ln\u00EDmi metodami. Studov\u00E1ny budou zejm\u00E9na autonomn\u00ED RNA moduly st\u0159edn\u00ED velikosti, tzv. RNA motivy. C\u00EDle jsou mj. l\u00E9pe pochopit pravidla, jimi\u017E se \u0159\u00EDd\u00ED kovariace b\u00E1z\u00ED v sekvenc\u00EDch RNA, vysv\u011Btlit, pro\u010D n\u011Bkter\u00E9 isosterick\u00E9 substituce nejsou b\u011Bhem evoluce t\u00E9m\u011B\u0159 nikdy realizov\u00E1ny a pro\u010D n\u011Bkdy doch\u00E1z\u00ED k z\u00E1m\u011Bn\u00E1m funk\u010Dn\u00EDch motiv\u016F v homologn\u00EDch molekul\u00E1ch RNA. D\u00E1le klasifikovat vertik\u00E1ln\u00ED interakce b\u00E1z\u00ED v RNA se zahrnut\u00EDm energi\u00ED, katalogizovat strukturn\u011B-dynamick\u00E9 vlastnosti RNA motiv\u016F a zp\u0159esnit 2D predikce intern\u00EDch RNA smy\u010Dek." . "Study of the basic principles how molecular interactions influence folding and function of large RNAs, such as the ribosome. The research will be conducted using state of the art computational (explicit solvent simulations and quantum chemistry) and bioinformatics methods, in a close cooperation with experimental laboratories. It aims primarily at analysis of energies of molecular interactions and description of stochastic flexibilities of basic RNA building blocks. Both features are crucial for function of RNAs but are very difficult to assess by experiments. The research will mainly be directed to key medium-sized autonomous RNA building blocks, with the aim to rationalize covariation principles in RNA sequences, explain lack of certain isosteric substitutions during evolution, analyze motif swaps in homologous RNAs, classify RNA base stacking patterns with inclusion of the stacking energies, catalogue structural dynamics of RNA motifs and improve 2D predictions of internal RNA loops. \u00A0"@en . " pairs" . . " and" . . . " quantum" . "Structural dynamics, molecular interactions and function of key RNA motifs"@en . " ribosome" . . . "Strukturn\u00ED dynamika, molekulov\u00E9 interakce a funkce kl\u00ED\u010Dov\u00FDch motiv\u016F RNA" . . " function" . . . "2014-01-30+01:00"^^ . . " bioinformatics" . . " dynamics" . . " molecular" . . . " base" . "http://www.isvav.cz/projectDetail.do?rowId=GA203/09/1476"^^ . "structure; and; function; of; RNA; molecular; dynamics; quantum; chemistry; bioinformatics; ribosome; base; pairs; RNA; motifs"@en . . . "2012-12-31+01:00"^^ . "1"^^ . "39"^^ . " RNA" . "According to the main goal of the project, the work significantly contributed to an improvement of the knowledge of RNA modular building blocks, the physical chemistry of their molecular interactions, and their inclusion in larger RNA systems.The project was extremely successful. This is also documented by 49 publications in impacted journals."@en . "39"^^ . "GA203/09/1476" . "2012-03-30+02:00"^^ .