"Development and application of electrochemical methods and biosensors in studies of nucleic acids structure and interactions and/or in detection of DNA hybridization or damage belongs to the most dynamically developing areas of contemporary science. In this project, we propose to continue the existing fruitful research in the Applicant's and Joint Applicant's laboratories and to obtain new knowledge about the behavior of unmodified and chemically modified nucleic acids, their complexes with low molecular mass genotoxic substances and/or with proteins at different electrodes, including newly introduced types of solid amalgam electrodes. The proposed research will involve detailed studies of electrochemical processes underwent by nucleic acids and their conjugates with electroactive markers at different electrode materials. Electrochemical analysis will be combined with separation techniques and with immunoanalysis to develop new methods of detection of DNA damage, its chemical modification"@en . "\u0158e\u0161en\u00ED projektu prob\u00EDhalo zcela bez probl\u00E9m\u016F, bylo dosa\u017Eeno v\u0161ech vyt\u010Den\u00FDch c\u00EDl\u016F a o \u00FAsp\u011B\u0161nosti projektu sv\u011Bd\u010D\u00ED i velmi nadstandardn\u00ED publika\u010Dn\u00ED aktivita \u0159e\u0161itelsk\u00E9ho t\u00FDmu. Celkov\u011B lze \u0159e\u0161en\u00ED projektu hodnotit jako vynikaj\u00EDc\u00ED."@cs . "0"^^ . . "Analysis of DNA structure and interactions using electrochemical techniques and chemical probes. Novel techniques and sensors for DNA damage detection"@en . " interactions" . " DNA structure" . . . "1"^^ . "1"^^ . "V\u00FDvoj a vyu\u017Eit\u00ED elektrochemick\u00FDch metod a biosensor\u016F p\u0159i studiu struktury a interakc\u00ED nukleov\u00FDch kyselin a jako n\u00E1stroj\u016F pro detekci hybridizace DNA a jej\u00EDho po\u0161kozen\u00ED pat\u0159\u00ED k nejdynami\u010Dt\u011Bji se rozv\u00EDjej\u00EDc\u00EDm oblastem sou\u010Dasn\u00E9 v\u011Bdy. V r\u00E1mci tohoto projektunavrhujeme nav\u00E1zat na dosavadn\u00ED \u00FAsp\u011B\u0161n\u00FD v\u00FDzkum v laborato\u0159\u00EDch navrhovatele i spolunavrhovatele a z\u00EDskat nov\u00E9 poznatky o chov\u00E1n\u00ED nemodifikovan\u00FDch a chemicky modifikovan\u00FDch nukleov\u00FDch kyselin, jejich komplex\u016F s n\u00EDzkomolekul\u00E1rn\u00EDmi genotoxick\u00FDmi l\u00E1tkami a s proteiny na r\u016Fzn\u00FDch typech elektrod, v\u010Detn\u011B nov\u011B zaveden\u00FDch typ\u016F pevn\u00FDch amalg\u00E1mov\u00FDch elektrod. Sou\u010D\u00E1st\u00ED navrhovan\u00E9ho v\u00FDzkumu je detailn\u00ED studium elektrochemick\u00FDch proces\u016F, jim\u017E podl\u00E9haj\u00ED na elektrod\u00E1ch z r\u016Fzn\u00FDch materi\u00E1l\u016F nukleov\u00E9 kyseliny a jejich konjug\u00E1ty s elektroaktivn\u00EDmi zna\u010Dkami. Elektrochemick\u00E1 anal\u00FDza bude kombinov\u00E1na se separa\u010Dn\u00EDmi technikami a s imunoanal\u00FDzou, a na z\u00E1klad\u011B t\u00E9to kombinace budou vyv\u00EDjeny nov\u00E9 metody detekce po\u0161kozen\u00ED DNA a jej\u00ED chemick\u00E9 modifikace in vitro i v bu\u0148k\u00E1ch, a" . "http://www.isvav.cz/projectDetail.do?rowId=GA203/07/1195"^^ . " genotoxic agents" . . . "."@en . " DNA damage" . "GA203/07/1195" . . . "88"^^ . . "88"^^ . . . "electrochemical analysis; DNA damage; genotoxic agents; interactions; DNA structure; chemical probes"@en . . "Anal\u00FDza struktury a interakc\u00ED DNA pomoc\u00ED elektrochemick\u00FDch technik a chemick\u00FDch sond. Nov\u00E9 metody a senzory pro detekci po\u0161kozen\u00ED DNA" . . . . "2015-01-22+01:00"^^ . . . "electrochemical analysis" . "2007-01-01+01:00"^^ . . . . "2009-12-31+01:00"^^ . "2009-04-22+02:00"^^ . .