"2009-10-22+02:00"^^ . "10"^^ . "Tolerance po\u0161kozen\u00ED DNA protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDmi komplexy kov\u016F" . "10"^^ . . "Tolerance and bypass of DNA damage by metal-based anticancer drugs"@en . . "antitumor drugs" . . . . . . . "This project proposes to examine the effect of DNA conformation on the catalytic efficiency and fidelity of DNA-dependent DNA and RNA polymerases on DNA templates containing site-specific adducts of various antitumor platinum and ruthenium compounds and their model analogs. The long-term goals are to understand the mechanism underlying resistance to metal-based drugs and their antitumor and mutagenic affects. In addition, the methodology based on the use of DNA and RNA polymerases and DNA templates globally modified by platinum or ruthenium antitumor\u00A0 compounds has been used in the %22replication%22 or %22transcription%22 mapping experiments to identify the arrest sites of DNA replication or transcription and consequently also preferential DNA binding sites of antitumor metal-based compounds. Thus, another long-term goal of our research is to improve theoretical background for the replication and transcription mapping of the lesions formed in DNA by the compounds of biological significance."@en . . "2006-01-01+01:00"^^ . "\u0158e\u0161en\u00EDm tohoto projektu byl objasn\u011Bn vliv konformace DNA na katalytickou \u00FA\u010Dinnost DNA-dependentn\u00EDch DNA a RNA polymer\u00E1z s vyu\u017Eit\u00EDm templ\u00E1t\u016F DNA obsahuj\u00EDc\u00EDch m\u00EDstn\u011B specifick\u00E9 adukty r\u016Fzn\u00FDch protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDch slou\u010Denin platiny a ruthenia a jejich mod"@cs . . "http://www.isvav.cz/projectDetail.do?rowId=GA203/06/1239"^^ . . . "antitumor drugs; DNA; cancer; resistance"@en . . "2008-04-25+02:00"^^ . . " DNA" . . "This project resolved the effect of DNA conformation on the catalytic efficiency and fidelity of DNA-dependent DNA and RNA polymerases on DNA templates containing site-specific adducts of various antitumor platinum and ruthenium compounds and their model"@en . . "0"^^ . "2008-12-31+01:00"^^ . " cancer" . . "Podstatou tohoto projektu je v\u00FDzkum vlivu konformace DNA na katalytickou \u00FA\u010Dinnost DNA-dependentn\u00EDch DNA a RNA polymer\u00E1z s vyu\u017Eit\u00EDm templ\u00E1t\u016F DNA obsahuj\u00EDc\u00EDch m\u00EDstn\u011B specifick\u00E9 adukty r\u016Fzn\u00FDch protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDch slou\u010Denin platiny a ruthenia a jejich modelov\u00FDch analog\u016F. Dlouhodob\u00E9 c\u00EDle takto orientovan\u00E9ho v\u00FDzkumu jsou porozum\u011Bt mechanism\u016Fm rezistence u farmak odvozen\u00FDch od komplex\u016F kov\u016F a jejich protin\u00E1dorov\u00FDm a mutagenn\u00EDm efekt\u016Fm. Krom\u011B toho metodologie zalo\u017Een\u00E9 na vyu\u017Eit\u00ED DNA a RNA polymer\u00E1z na templ\u00E1tech DNA glob\u00E1ln\u011B modifikovan\u00FDch protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDmi slou\u010Deninami platiny a ruthenia jsou\u00A0 vyu\u017E\u00EDv\u00E1ny p\u0159i replika\u010Dn\u00EDm a transkrip\u010Dn\u00EDm mapov\u00E1n\u00ED\u00A0 m\u00EDst v DNA, ve kter\u00FDch doch\u00E1z\u00ED k zastaven\u00ED replikace nebo transkripce DNA a tedy tak\u00E9 m\u00EDst, ke kter\u00FDm se v DNA preferen\u010Dn\u011B v\u00E1\u017E\u00ED protin\u00E1dorov\u011B \u00FA\u010Dinn\u00E9 komplexy kov\u016F. Dal\u0161\u00EDm dlouhodob\u00FDm c\u00EDlem na\u0161eho v\u00FDzkumu je tedy tak\u00E9 zdokonalit teoretick\u00E9 z\u00E1zem\u00ED pro replika\u010Dn\u00ED a transkrip\u010Dn\u00ED mapov\u00E1n\u00ED m\u00EDst v DNA po\u0161kozen\u00FDch biologicky v\u00FDznamn\u00FDmi l\u00E1tkami." . "1"^^ . . . "0"^^ . . "GA203/06/1239" .