. . "The objective of the present project was to study the structural changes of DNA modified by platinum and rutheniun cytostatics by different biophysical methods and to confirm sensitivity of laser Raman spectroscopy to detect structural changes induced in"@en . . . . "GA203/05/2032" . . . . "Raman spectroscopy of DNA modified by antitumor metal-based compounds"@en . . "Raman spectroscopy; metal based drugs; cancer"@en . "Od objevu protin\u00E1dorov\u00E9 \u00FA\u010Dinnosti cisplatiny \u017E\u00E1dn\u00FD z jej\u00EDch p\u0159\u00EDm\u00FDch analog\u016F, kter\u00FD byl klinicky testov\u00E1n, nevykazuje klinick\u00E9 vlastnosti, znamenaj\u00EDc\u00ED z\u00E1sadn\u00ED pr\u016Flom v chemoterapii n\u00E1dor\u016F cytostatiky odvozen\u00FDmi od komplex\u016F kov\u016F. Z tohoto d\u016Fvodu pokra\u010Duje intenzivn\u00ED vyhled\u00E1v\u00E1n\u00ED lep\u0161\u00EDch cytostatik na b\u00E1zi komplex\u016F kov\u016F motivovan\u00E9 pot\u0159ebou navrhnout m\u00E9n\u011B toxickou l\u00E1tku, kter\u00E1 by nevykazovala k\u0159\u00ED\u017Eovou resistenci s cisplatinou nebo jej\u00EDmi p\u0159\u00EDm\u00FDmi analogy. P\u0159i tomto vyhled\u00E1v\u00E1n\u00ED je testov\u00E1na hypot\u00E9za, podle kter\u00E9 ty komplexy kov\u016F, kter\u00E9 se v\u00E1\u017E\u00ED k DNA zp\u016Fsobem z\u00E1sadn\u011B odli\u0161n\u00FDm od modifikac\u00ED DNA cisplatinou, budou m\u00EDt odli\u0161n\u00E9 farmakologick\u00E9 vlastnosti. Konformace DNA modifikovan\u00E9 cytostatiky na b\u00E1zi komplex\u016F kov\u016F ji\u017E byla studov\u00E1na pomoc\u00ED \u0159ady technik molekul\u00E1rn\u00ED biologie a biofyziky. Av\u0161ak zcela neo\u010Dek\u00E1van\u011B pouze mal\u00E1 pozornost byla dosud v\u011Bnov\u00E1na anal\u00FDze DNA modifikovan\u00E9 t\u00EDmto typem cytostatik pomoc\u00ED Ramanovy spektroskopie p\u0159esto, \u017Ee pou\u017Eit\u00ED t\u00E9to techniky m\u016F\u017Ee poskytnout zcela unik\u00E1tn\u00ED informace. V" . "2008-12-16+01:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA203/05/2032"^^ . "Since discovery of antitumor activity of cisplatin, none of its direct structural analogues which h\u00E1ve advanced to clinical trials are likely to display clinical properties that would be genuinely novel in comparison to the parent drug. Therefore, the search continues for an improved metal-based antitumor agent, motivated by the desire to design a Iess toxic compound that is non-cross-resistant with cisplatin or its direct analogs. In this search the hypothesis that platinum drugs which bind to DNA ina fundamentally different manner to that of cisplatin will h\u00E1ve altered pharmacological properties has been tested. Conformation of DNA modified by metal-based drugs has been studied by a large number of various techniques of molecular biophysics and biology. However, quite surprisingly only a Iittle attention has been paid to analysis of DNA modified by metal-based antitumor drugs by Raman spectroscopy in spite of the unique information that can be obtained using this technique. This application"@en . . . . "2007-12-31+01:00"^^ . . . "4"^^ . "0"^^ . "4"^^ . "2005-01-01+01:00"^^ . . "Ramanova spektroskopie DNA modifikovan\u00E9 protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDmi komplexy kov\u016F" . "1"^^ . "C\u00EDlem tohoto projektu bylo s vyu\u017Eit\u00EDm r\u016Fzn\u00FDch biofyzik\u00E1ln\u00EDch metod studovat strukturn\u00ED zm\u011Bny v DNA, kter\u00E1 byla modifikov\u00E1na platinov\u00FDmi a rutheniov\u00FDmi cytostatiky a potvrdit citlivost Ramanovy spektroskopie k detekci strukturn\u00EDch zm\u011Bn vyvolan\u00FDch v DNA pl"@cs . . "Raman spectroscopy" . . . "2007-05-02+02:00"^^ . . . " metal based drugs" . "0"^^ .