"GA203/05/0043" . " sensors" . "2007-12-31+01:00"^^ . " electrochemistry" . . . . . . . . "1"^^ . "Projekt bude zam\u011B\u0159en na synt\u00E9zu nov\u00FDch typ\u016F konjug\u00E1t\u016F purinov\u00FDch a pyrimidinov\u00FDch b\u00E1z\u00ED a nukleosid\u016F s komplexy kov\u016F (nap\u0159. ferrocen, komplexy bipyridinu \u010Di fenanthrolinu s r\u016Fzn\u00FDmi p\u0159echodn\u00FDmi kovy atd.) spojen\u00FDch p\u0159es konjugovan\u00FD linker. Takto ozna\u010Den\u00E9 nukleosidy budou p\u0159evedeny na chr\u00E1n\u011Bn\u00E9 fosforamidity a pou\u017Eity v synt\u00E9ze oligonukleotid\u016F. Bude studov\u00E1no elektrochemick\u00E9 chov\u00E1n\u00ED zna\u010Den\u00FDch oligonukletid\u016F v jejich jedno\u0159et\u011Bzcov\u00E9 a dvou\u0159et\u011Bzcov\u00E9 form\u011B (v\u010Detn\u011B duplex\u016F s \u0159et\u011Bzci obsahuj\u00EDc\u00EDmi bodov\u00E9 mutace).C\u00EDlem bude naj\u00EDt syst\u00E9m, jeho\u017E elektrochemick\u00E9 vlastnosti budou v\u00FDrazn\u011B ovlivn\u011Bny tvorbou duplexu DNA, a vyu\u017E\u00EDt jej p\u0159i v\u00FDvoji senzor\u016F pro hybridizaci DNA. P\u0159edpokl\u00E1d\u00E1me, \u017Ee se vliv interakc\u00ED mezi p\u00E1ry baz\u00ED v dvou\u0161roubovici DNA p\u0159enese p\u0159es konjugovan\u00FD syst\u00E9m na marker (komplex kovu). Potenci\u00E1ln\u00ED markery budou tak\u00E9 studov\u00E1ny teoretick\u00FDmi v\u00FDpo\u010Detn\u00EDmi metodami a vypo\u010Dten\u00E9 ioniza\u010Dn\u00ED potenci\u00E1ly srovn\u00E1v\u00E1ny s experiment\u00E1ln\u00EDmi daty. V\u00FDpo\u010Dty budou vyu\u017Eity p\u0159i racion\u00E1ln\u00EDm designu nov\u00FDch marker\u016F." . "1"^^ . . "2005-01-01+01:00"^^ . . "2007-05-02+02:00"^^ . "0"^^ . " oligonucleotides" . "nucleobases" . "15"^^ . "15"^^ . . . . "Konjug\u00E1ty nukleob\u00E1z\u00ED s komplexy kov\u016F jako elektroaktivn\u00ED zna\u010Dky. Vyu\u017Eit\u00ED zna\u010Den\u00FDch oligonukleotid\u016F v senzorech pro hybridizaci DNA" . " nucleotides" . . . "nucleobases; nucleotides; oligonucleotides; metal complexes; electrochemistry; sensors; DNA"@en . . . . . "Conjugates of nucleobases with metal complexes as electroactive markers. Application of labelled oligonucleotides in electrochemical DNA sensors"@en . . "C\u00EDle projektu byly spln\u011Bny a zna\u010Dn\u011B roz\u0161\u00ED\u0159eny. Byla vypracov\u00E1na metodika arylac\u00ED a alkynylac\u00ED nukleob\u00E1z\u00ED a nukleosid\u016F cross-coupling reakcemi a synt\u00E9za konjug\u00E1t\u016F nukleob\u00E1z\u00ED a nukleosid\u016F s Ru a Os komplexy. P\u016Fvodn\u00ED z\u00E1m\u011Br p\u0159ipravovat zna\u010Den\u00E9 oligonukleotid"@cs . "http://www.isvav.cz/projectDetail.do?rowId=GA203/05/0043"^^ . " metal complexes" . "The goals of the project have been fulfilled and significantly extended. Methodology for arylation and alkynylation of nucleobases and nucleosides by cross-coupling reactions and synthesis of conjugates of nucleobases and nucleosides with Ru and Os compl"@en . "The project will deal with the synthesis of novel types of conjugates of purine and pyrimidine bases with metal complexes (e.g. ferrocene, transition metal complexes of bipyridine or phenanthroline etc.) connected by a conjugated linker. Such labelled nucleosides will be transformed to protected phosphoramidites and use in oligonucleotide synthesis. We will study electrochemical behaviour of the labelled oligonucleotides in their single-stranded as well as double-stranded forms (including duplexes involving single-base mismatches). The goal is to find a system whose electrochemical features will be significantly affected by the duplex formation. Such system will be utilized in development of sensors for DNA hybridization. We suppose that effects of interactions between base pairs in the DNA double helix will be transferred through the conjugated system to the marker (metal complex). Potential markers will be also studied by theoretical chemistry calculations and the calculated ionization"@en . . "2008-12-16+01:00"^^ .