. . . "16"^^ . . "In this project, a new knowledge on the syntheses of protected and free peptides of the hinge sequence of human immunoglobulin IgG1 has been obtained. We optimized three synthetic approaches based on solution and solid phase synthesis, utilizing a solubl"@en . . "Neuvedeno."@en . . "0"^^ . "GA203/03/1362" . "2009-01-15+01:00"^^ . "0"^^ . . . . "http://www.isvav.cz/projectDetail.do?rowId=GA203/03/1362"^^ . "Synthesis and characterization of synthetic antigens"@en . "1"^^ . . . "Within development of new synthetic vaccines of peptidic nature we will synthesize the %22hinge peptide%22 which is a parallel dimer of the octapeptide H-Thr-Cys(..)-Pro-Pro.Cys(..)-Pro-Ala-Pro-OH - the IgGl fragment - and which has already been usedpreviously with succes as antigenic carrier in the preparation of antibodies agains minigastrin. We will optimize the synthesis of sufficient amounts in standart quality for the subsequent studies of coupling to antigens, immunological response andphysico-chemical properties. We will use differential protection of all four terminal groups and will elaborate an optimal dimerization procedure with respect to possible variations when preparing substituted derivatives. As a model system we will usesequences which are antigenic against Plasmodium falciparum merozoites and sporozoites, respectively. It has been shown on this system already, that a synthetic peptidic antigen can be prepared and used as a vaccine. It is an attractive possibility that"@en . . "V r\u00E1mci v\u00FDvoje nov\u00FDch syntetick\u00FDch vakc\u00EDn peptidov\u00E9 povahy se zam\u011B\u0159\u00EDme na p\u0159\u00EDpravu %22hinge peptidu%22, kter\u00FD je paraleln\u00EDm dimerem oktapeptidu H-Thr-Cys(..)-Pro-Pro.Cys(..)-Pro-Ala-Pro-OH - fragmentu imunoglobulinu IgGl - a kter\u00FD byl ji\u017E v minulosti \u00FAsp\u011B\u0161n\u011Bpou\u017Eit jako nosi\u010D antigenu p\u0159i p\u0159\u00EDprav\u011B protil\u00E1tek proti minigastrinu. Vypracujeme optim\u00E1ln\u00ED variantu synt\u00E9zy v dostate\u010Dn\u00E9m mno\u017Estv\u00ED a standardn\u00ED kvalit\u011B pro pozd\u011Bj\u0161\u00ED studia vazby antigenu, imunologick\u00E9 odpov\u011Bdi a fyzik\u00E1ln\u011B-chemick\u00FDch vlastnost\u00ED.Pou\u017Eijeme diferen\u010Dn\u00ED chr\u00E1n\u011Bn\u00ED v\u0161ech \u010Dty\u0159 termin\u00E1ln\u00EDch skupin a vypracujeme nejvhodn\u011Bj\u0161\u00ED postup dimerizace s ohledem na mo\u017Enosti variace postupu pro z\u00EDsk\u00E1n\u00ED substituovan\u00FDch deriv\u00E1t\u016F. Jako modelov\u00FD syst\u00E9m pou\u017Eijeme polypeptidov\u00E9 sekvence antigenn\u00EDmerozoit\u016Fm a sporozoit\u016Fm Plasmodium falciparum, na kter\u00FDch ji\u017E bylo uk\u00E1z\u00E1no, \u017Ee syntetick\u00E1 p\u0159\u00EDprava peptidov\u00E9ho antigenu pro pou\u017Eit\u00ED jako vakc\u00EDna je mo\u017En\u00E1. P\u0159i pou\u017Eit\u00ED %22hinge peptidu%22 jako z\u00E1kladn\u00EDho nosi\u010De se ukazuje atraktivn\u00ED mo\u017Enost pracovat s" . . . . . . . . "Synt\u00E9za a charakterizace syntetick\u00FDch antigen\u016F" . "P\u0159i \u0159e\u0161en\u00ED projektu byly z\u00EDsk\u00E1ny nov\u00E9 poznatky o synt\u00E9ze chr\u00E1n\u011Bn\u00FDch i voln\u00FDch peptid\u016F z hinge sekvence lidsk\u00E9ho immunoglobulinu IgG1. Optimalizovali jsme t\u0159i syntetick\u00E9 postupy zalo\u017Een\u00E9 na synt\u00E9ze v roztoku i na pevn\u00E9 f\u00E1zi vyu\u017E\u00EDvaj\u00EDc\u00ED jak rozpustn\u00FD PEG n"@cs . "16"^^ .