. . "Srovn\u00E1n\u00ED sekvenc\u00ED DNA polyvalentn\u00EDho stafylokokov\u00E9ho f\u00E1ga 812, jeho mutant a p\u0159\u00EDbuzn\u00FDch f\u00E1g\u016F vedlo k odhalen\u00ED a n\u00E1sledn\u011B pak k podrobn\u00E9 charakterizaci muta\u010Dn\u00EDch zm\u011Bn f\u00E1gov\u00E9ho genomu, maj\u00EDc\u00EDch p\u0159\u00EDmou souvislost s jejich rozmez\u00EDm hostitel\u016F. V\u00FDznamn\u00FDm v\u00FDsle"@cs . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA203/03/0515"^^ . . . . "1"^^ . . . . "1"^^ . . "P\u0159edpokl\u00E1d\u00E1 se, \u017Ee z\u00E1stupci n\u011Bkter\u00FDch kmen\u016F bakteriof\u00E1g\u016F (vir\u016F bakteri\u00ED), p\u0159\u00EDpadn\u011B jejich specifick\u00E9 proteiny a peptidy, mohou slou\u017Eit jako nov\u00E9 \u00FA\u010Dinn\u00E9 prost\u0159edky v l\u00E9\u010Db\u011B bakteri\u00E1ln\u00EDch infekc\u00ED. Pro racion\u00E1ln\u00ED v\u00FDvoj f\u00E1gov\u00E9 terapie, bez ne\u017E\u00E1douc\u00EDch \u00FA\u010Dink\u016Fsou\u010Dasn\u00FDch antibiotik, je t\u0159eba nal\u00E9zt vztah mezi po\u017Eadovan\u00FDmi biologick\u00FDmi vlastnostmi vhodn\u00FDch f\u00E1g\u016F a jejich genomem a proteomem. K dosa\u017Een\u00ED tohoto c\u00EDle je nutn\u00E9 vyvinout novou technologii pro rychlou a spolehlivou anal\u00FDzu f\u00E1gov\u00E9 molekul\u00E1rn\u00ED struktury.V tomto projektu bude vyvinut trojrozm\u011Brn\u00FD separa\u010Dn\u00ED syst\u00E9m pro anal\u00FDzu f\u00E1g\u016F. V prvn\u00ED f\u00E1zi pou\u017Eijeme pro separaci celkov\u00E9 sm\u011Bsi f\u00E1gov\u00FDch protein\u016F mikropreparativn\u00ED kapil\u00E1rn\u00ED elektrofor\u00E9zu s vysoce citlivou detekc\u00ED na b\u00E1zi laserem indukovan\u00E9 fluorescence(LIF) s nekovalentn\u00EDm zna\u010Den\u00EDm. Z\u00EDskan\u00FD fingerprint poslou\u017E\u00ED k odhalen\u00ED diferenc\u00ED proteinov\u00E9ho obsahu f\u00E1g\u016F a k \u0159\u00EDzen\u00ED automatick\u00E9ho sb\u011Bru frakc\u00ED. Frakce bodou n\u00E1sledn\u011B \u0161t\u011Bpeny trypsinem imobilizovan\u00FDm na st\u011Bn\u00E1ch sb\u011Brn\u00FDch jamek. V z\u00E1v\u011Bre\u010Dn\u00E9m kroku budou" . . . . "2009-01-15+01:00"^^ . . "38"^^ . . . "38"^^ . "Comparison of DNA sequences of polyvalent staphylococcal phage 812, its mutants and related phages led to discovery and consequently to detail characterization of mutation changes in phage genome, which are related directly to their host range. Major res"@en . . "It is expected that selected bacteriophages (bacterial viruses), or even their specific proteins and/or peptides can be used as new effective means to treat bacterial infections. For a rational development of phage therapy, without the adverse effects ofcurrent antibiotics, the relationship between desired biological properties of suitable phages and their genome/proteome structure must be found. To achieve this goal it is necessary to find new technologies for rapid analysis of the phage molecularstructure. In this project, we will develop a three-dimensional separation system for analysis and typing of the phage proteome. First, micropreparative capillary electrophoresis with laser-induced fluorescence detection (LIF) and noncovalent labelingwill be used for the separation of the phage proteins. This fingerprint will be used to trace differences in protein content of phages and to control the automated fraction collection. The resulting fractions will be digested by trypsin immobilized"@en . . "Neuvedeno."@en . "GA203/03/0515" . "Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry"@en . "Integrovan\u00E1 anal\u00FDza genomu a proteomu terapeuticky v\u00FDznamn\u00FDch bakteriof\u00E1g\u016F kombinac\u00ED elektrofor\u00E9zy a hmotnostn\u00ED spektrometrie" . . . .