. . "3"^^ . . . . "0"^^ . . "3"^^ . . "Projekt p\u0159ed\u010Dasn\u011B ukon\u010Den z d\u016Fvodu ukon\u010Den\u00ED pracovn\u00EDho pom\u011Bru \u0159e\u0161itele - dlouhodob\u00FD zahrasni\u010Dn\u00ED pobyt. Administrativn\u00ED str\u00E1nka projektu byla v souladu s grantov\u00FDmi pravidly. Z \u0159e\u0161en\u00ED je z\u0159ejm\u00E9, \u017Ee z\u00E1m\u011Bry projektu pl\u00E1novan\u00E9 na jeho prvn\u00ED rok byly re\u00E1ln\u00E9 a"@cs . . "1"^^ . "GA203/00/P038" . . "0"^^ . . . "Determination of D-aminoacids in proteins of selected human tissues"@en . "Stanoven\u00ED D-aminokyselin v proteinech vybran\u00FDch lidsk\u00FDch tk\u00E1n\u00ED" . "http://www.isvav.cz/projectDetail.do?rowId=GA203/00/P038"^^ . . . "Occurence, origin and function of D-aminoacids (DAMAs) in the proteins are not yet fully understood.The DAMAs have been detected in the human enamel, dentin, erythrocytes, white brain matter, myelin end eye lens. Anomalous occurrence of DAMAs can serve as an indicator for some pathogenic metabolism disturbances. An analytical method for the determination of low DAMAs concentration levels in the selected human tissues is an essential condition for further investigation of this subject. Published methodseither do nat allow separation of all physiological aminoacids or use complicated sample preparation or are not suficiently sensitive and reproducible or do not consider the aminoacid racemization during the sample preparation and analysis. The goal of the project is to elaborate a sensitive and precise analytical method for the determination of DAMAs in proteins of the human tissues (dentin, bone, cartilage, tendon, vitreous body, white brain matter), anoptimization of the sample preparation condition"@en . . . . "V\u00FDskyt, p\u016Fvod a funkce D-forem aminokyselin (DAMK) v proteinech jsou doposud zna\u010Dn\u011B nejasn\u00E9. U lid\u00ED byl zji\u0161t\u011Bn v\u00FDskyt DAMK v zubn\u00ED sklovin\u011B, dentinu, erytrocytech, b\u00EDl\u00E9 hmot\u011B mozkov\u00E9, myelinu a o\u010Dn\u00ED \u010Do\u010Dce. Abnorm\u00E1ln\u00ED v\u00FDskyt DAMK m\u016F\u017Ee slou\u017Eit tak\u00E9 jako indik\u00E1tor patogenn\u00EDch poruch metabolismu doprov\u00E1zej\u00EDc\u00ED n\u011Bkter\u00E1 onemocn\u011Bn\u00ED. Z\u00E1kladn\u00EDm p\u0159edpokladem nezbytn\u00FDm pro v\u00FDzkum v t\u00E9to oblasti je analytick\u00E1 metodologie stanoven\u00ED velmi n\u00EDzk\u00FDch koncentrac\u00ED DAMK ve zkouman\u00FDch tk\u00E1n\u00EDch. Publikovan\u00E9 metody neumo\u017E\u0148uj\u00ED stanoven\u00ED v\u0161ech biogenn\u00EDch DAMK, pou\u017E\u00EDvaj\u00ED komplikovan\u00E9 metody \u00FApravy vzork\u016F, nedosahuj\u00ED dostate\u010Dn\u00E9 citlivosti a reprodukovatelnosti a neuva\u017Euj\u00ED vliv racemizace aminokyselin v pr\u016Fb\u011Bhu \u00FApravy vzorku a jeho anal\u00FDzy. C\u00EDlem grantu je vypracov\u00E1n\u00ED p\u0159esn\u00E9 a velmi citliv\u00E9 metody stanoven\u00ED DAMK v proteinech lidsk\u00FDch tk\u00E1n\u00ED (dentin, kost, chrupavka, \u0161lacha, sklivec, b\u00EDl\u00E1 hmota mozkov\u00E1), nalezen\u00ED optim\u00E1ln\u00EDch podm\u00EDnek p\u0159\u00EDpravy vzork\u016F jednotliv\u00FDch tk\u00E1n\u00ED, optimalizace pracovn\u00EDch podm\u00EDnek anal\u00FDzy a validace metody p" .