"0"^^ . "Project is aimed at the design of new hydrophilic magnetic carriers of spherical shape and uniform size in range of micrometers, which can be used in biotechnological applications. The carriers will be prepared by dispersion polymerization in the presencof various magnetic iron oxides. The effect of type and amount of iron oxide added, solvent mixture, monomer concentration, type and concentration of stabilizer and initiator and temperature on the properties of the resulting particles will be determinedand the polymerization mechanism elucidated. Various affinity ligands, such as enzymes, antibodies, or reactive dyes will be bound to modified magnetic particles. For example, RNase, proteinase, lysozyme, or antibodies against some pathogenic bacteria will be immobilized on the magnetic particles. Activity of immobilized proteins will be tested in the preparation of crude cell lysate, or by means of immunomagnetic separation. Particles with immobilized bacteria will be used in polymerase chain"@en . "Projekt d\u00E1le rozv\u00EDjej\u00EDc\u00ED d\u0159\u00EDv\u011Bj\u0161\u00ED projekty. Byla vyvinuta a studov\u00E1na p\u0159\u00EDprava magnetick\u00FDch mikro\u010D\u00E1stic s \u00FAzkou distribuc\u00ED velikost\u00ED na podklad\u011B vyu\u017Eit\u00ED disperzn\u00ED polymerizace hydrofiln\u00EDch monomer\u016F (p\u0159edev\u0161\u00EDm HEMA a GDA),magnetick\u00E9 mikro\u010D\u00E1stice byly p\u0159ip"@cs . "Dispersion polymerisation of hydrophilic monomers for the preparation of magnetic carriers used in biotechnological application"@en . "25"^^ . "25"^^ . "C\u00EDlem projektu je vyvinout nov\u00E9 hydrofiln\u00ED magnetick\u00E9 nosi\u010De sf\u00E9rick\u00E9ho tvaru a jednotn\u00E9 velikosti \u0159\u00E1dov\u011B v mikrometrech pro biotechnologick\u00E9 aplikace. Nosi\u010De budou p\u0159ipravov\u00E1ny disperzn\u00ED polymerizac\u00ED v p\u0159\u00EDtomnosti r\u016Fzn\u00FDch magnetick\u00FDch oxid\u016F \u017Eeleza. Budestudov\u00E1n vliv typu a mno\u017Estv\u00ED oxidu \u017Eeleza, rozpou\u0161t\u011Bdlov\u00E9 sm\u011Bsi, koncentrace monomer\u016F, typu a koncentrace stabiliz\u00E1toru a inici\u00E1toru a teploty na vlastnosti v\u00FDsledn\u00FDch \u010D\u00E1stic a objasn\u011Bn mechanizmus polymerizace. Na modifikovan\u00E9 magnetick\u00E9 \u010D\u00E1stice budou nav\u00E1z\u00E1ny r\u016Fzn\u00E9 afinitn\u00ED ligandy, jako jsou enzymy, protil\u00E1tky nebo reaktivn\u00ED barviva, nap\u0159. RNasa, proteinasa, lysozym \u010Di protil\u00E1tky proti n\u011Bkter\u00FDm patogenn\u00EDm bakteri\u00EDm. Aktivita imobilizovan\u00FDch b\u00EDlkovin bude testov\u00E1na p\u0159i p\u0159\u00EDprav\u011B hrub\u00E9ho lyz\u00E1tu bun\u011Bk, pop\u0159. pomoc\u00ED imunomagnetick\u00E9 separace. \u010C\u00E1stice s imobilizovan\u00FDmi bakteriemi budou pou\u017Eity v polymer\u00E1zov\u00E9 \u0159et\u011Bzov\u00E9 reakci. Disperzn\u00ED polymerac\u00ED budou p\u0159ipraveny nejen nov\u00E9 hydrofiln\u00ED magnetick\u00E9 mikro\u010D\u00E1stice, ale i nosi\u010De na b\u00E1zi styrenu a divinylbenzenu." . . . "Disperzn\u00ED polymerizace hydrofiln\u00EDch monometr\u016F pro p\u0159\u00EDpravu magnetick\u00FDch nosi\u010D\u016F pou\u017E\u00EDvan\u00FDch v biotechnologick\u00FDch aplikac\u00EDch" . "GA203/00/1339" . . . . . . . "1"^^ . . . "1"^^ . "Neuvedeno."@en . . . . . . "2008-05-19+02:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA203/00/1339"^^ . .