. . "http://www.isvav.cz/projectDetail.do?rowId=GA203/00/0772"^^ . . . . "C\u00EDlem tohoto projektu je vyvinout instrumentaci a metodiku vysoce citliv\u00FDch a rychl\u00FDch anal\u00FDz biologicky v\u00FDznamn\u00FDch molekul, kter\u00E9 indikuj\u00ED n\u011Bkter\u00E1 z\u00E1va\u017En\u00E1 onemocn\u011Bn\u00ED. Hlavn\u00ED \u00FAsil\u00ED bude v\u011Bnov\u00E1no v\u00FDvoji metod pro prenat\u00E1ln\u00ED a t\u0159\u00EDd\u00EDc\u00ED diagnostiku, zalo\u017Een\u00FDch na detekci mutac\u00ED genomov\u00E9 DNA. Na\u0161e ned\u00E1vn\u00E9 zku\u0161enosti ukazuj\u00ED, \u017Ee z\u00E1kladn\u00EDm p\u0159edpokladem zv\u00FD\u0161en\u00ED citlivosti a rychlosti anal\u00FDz je implementace laserov\u00E9 spektroskopie jako detek\u010Dn\u00ED metody a miniaturizace za\u0159\u00EDzen\u00ED pro separaci. Elektroforetickseparace fragment\u016F DNA detekovan\u00FDch pomoc\u00ED laserem indukovan\u00E9 fluorescence m\u016F\u017Ee b\u00FDt realizov\u00E1na v kapil\u00E1r\u00E1ch o d\u00E9lce n\u011Bkolika centimetr\u016F nebo na \u010Dipech bez ztr\u00E1ty rozli\u0161en\u00ED. Takto lze zkr\u00E1tit dobu anal\u00FDzy d\u00E9lkov\u00E9ho nebo konforma\u010Dn\u00EDho polymorfismu DNA na dobu n\u011Bkolika v\u00FDzkumu analytick\u00E9 instrumentace a metodiky: 1. Detekce mutac\u00ED sekvenov\u00E1n\u00EDm kr\u00E1tk\u00FDch \u00FAsek\u016F genu pro enzym ATPasu. Deficitem tohoto enzymu doch\u00E1z\u00ED k z\u00E1va\u017En\u00E9 d\u011Bdi\u010Dn\u00E9 poru\u0161e metabolismu m\u011Bdi, Wilsonov\u011B chorob\u011B, kter\u00E1 je vysokou frekvenc\u00ED" . . . "Neuvedeno."@en . . "1"^^ . . . "Stanoven\u00E9 c\u00EDle byly spln\u011Bny s bohat\u00FDmi publika\u010Dn\u00EDmi v\u00FDstupy, o dosa\u017Een\u00FDch v\u00FDsledc\u00EDch bylo referov\u00E1no na \u010Detn\u00FDch mezin\u00E1rodn\u00EDch i dom\u00E1c\u00EDch konferenc\u00EDch. Nad r\u00E1mec \u00FAkol\u016F vyt\u00FD\u010Den\u00FDch v p\u0159ihl\u00E1\u0161ce projektu byla v\u011Bnov\u00E1na pozornost problematice multikapil\u00E1rn\u00ED pre"@cs . "2"^^ . . . . "2008-05-19+02:00"^^ . "0"^^ . "GA203/00/0772" . . "Development of ultrasensitive analytical methods for molecular identification and diagnostics of serious diseases"@en . . . "The overall goal of the project is to develop the instrumentation and methodology of high sensitive and fast analyses of biologically important molecules. The main effort will be focused on the development of methods for prenatal diagnostics and population screening, based on mutation detection in genomic DNA. Our latest experiments show that the prerequisites of sensitivity and analysis speed enhancements are the application of laser spectroscopy as a detection principle and miniaturization of the separation device. An electrophoretic separation of DNA fragments detected by laser induced fluorescence can be accomplished in short capillaries or on chips without a loss of resolution. Thus, the analysis time of length and conformational polymorphism of DNA can be shortened to several tens of seconds and a sequence read length of 400 nucleotides can be attained in few minutes. The submitted project includes three research areas of analytical instrumentation and methodology: l. Mutation"@en . "V\u00FDvoj ultracitliv\u00FDch analytick\u00FDch metod pro molekul\u00E1rn\u00ED identifikaci a diagnostiku z\u00E1va\u017En\u00FDch chorob" . "18"^^ . "18"^^ . .