" plasmon resonance" . "2007-05-02+02:00"^^ . "Raman" . "2005-01-01+01:00"^^ . "In accordance with the project aims, advanced techniques and experimental procedures have been developed and optimized for sensitive monitoring those interactions of nucleic acids (NA) that play important role in gene expression and its regulation in oli"@en . "Raman; plasmon resonance; sensor; nucleic acid; protein; antisense"@en . "2008-12-16+01:00"^^ . . "11"^^ . "Noninvasive physical monitoring of molecular interactions in regulation of gene expression"@en . "11"^^ . . . "2007-12-31+01:00"^^ . "Advanced optical methods capable to diagnose sensitively biomolecular interactions in small quantities without the interaction site labeling, will be developed and applied to study of nucleic acid - protein interactions, in particular those playing important role in regulation of gene expression and/or in %22antisense%22 therapy. Formation, structure and biochemical activity of enzyme - nucleic acid complexes in respect to the base sequence and/or nucleotide modification will be studied on selected cleavage and regulation enzymes. Methodological developments will comprise (i) technical and mathematical solution for precise Raman spectroscopy in nmol quantities, (ii) construction of high-resolution surface plasmon resonance sensor system with up to eight real-time observation channels, (iii) design of complex biomolecular interaction models considering effect of hydrodynamic conditions, (iv) preparation of structurally diverse sugar-phosphate backbone modified oligodeoxynucleotides."@en . . "V souladu s pl\u00E1novan\u00FDmi c\u00EDli projektu byla vyvinuta nov\u00E1 technika a metodick\u00E9 postupy pro citliv\u00E9 experiment\u00E1ln\u00ED sledov\u00E1n\u00ED interakc\u00ED nukleov\u00FDch kyselin (NA) v\u00FDznamn\u00FDch z hlediska genov\u00E9 exprese a jej\u00EDho c\u00EDlen\u00E9ho ovliv\u0148ov\u00E1n\u00ED p\u0159i oligonukleotidov\u00E9 terapii."@cs . . . " sensor" . "Neinvazivn\u00ED fyzik\u00E1ln\u00ED diagnostika molekul\u00E1rn\u00EDch interakc\u00ED p\u0159i regulaci genov\u00E9 exprese" . . . " nucleic acid" . . . . " protein" . . . "http://www.isvav.cz/projectDetail.do?rowId=GA202/05/0628"^^ . "1"^^ . "2"^^ . . . . "Budou rozvinut\u00E9 \u00FA\u010Dinn\u00E9 optick\u00E9 metody citliv\u00E9ho sledov\u00E1n\u00ED biomolekul\u00E1rn\u00EDch interakc\u00ED v mal\u00FDch objemech vzork\u016F bez nutnosti zav\u00E1d\u011Bn\u00ED molekul\u00E1rn\u00EDch zna\u010Dek do interak\u010Dn\u00EDho m\u00EDsta a budou aplikovan\u00E9 p\u0159i studiu t\u011Bch interakc\u00ED nukleov\u00E1 kyselina - protein, kter\u00E9hraj\u00ED v\u00FDznamnou roli v regulaci genov\u00E9 exprese a v %22antisense%22 terapii. U vybran\u00FDch \u0161t\u011Bp\u00EDc\u00EDch a regula\u010Dn\u00EDch enzym\u016F bude sledov\u00E1na tvorba, struktura a biochemick\u00E1 aktivita jejich komplex\u016F s nukleov\u00FDmi kyselinami. Metodick\u00FD v\u00FDvoj bude zahrnovat (i) technick\u00E9 a matematick\u00E9 \u0159e\u0161en\u00ED p\u0159esn\u00FDch m\u011B\u0159en\u00ED Ramanova rozptylu v nmol mno\u017Estv\u00EDch, (ii) konstrukci senzorov\u00E9ho syst\u00E9mu vysok\u00E9ho rozli\u0161en\u00ED s a\u017E osmi v re\u00E1ln\u00E9m \u010Dase m\u011B\u0159\u00EDc\u00EDmi kan\u00E1ly, zalo\u017Een\u00E9m na rezonanci povrchov\u00FDch plasmon\u016F, (iii) vypracov\u00E1n\u00ED komplexn\u00EDch interak\u010Dn\u00EDch model\u016F pro biomolekuly zahrnuj\u00EDc\u00EDch i vliv hydrodynamick\u00FDch podm\u00EDnek, (iv) p\u0159\u00EDpravu modifikovan\u00FDch oligonukleotid\u016F s r\u016Fznou strukturou cukr - fosf\u00E1tov\u00E9 p\u00E1te\u0159e." . . . . "0"^^ . "GA202/05/0628" . .