"GA202/04/0907" . . . . "High-resolution cytomery of living cells"@en . . . "1"^^ . . "B\u011Bhem \u0159e\u0161en\u00ED projektu byly v na\u0161ich laborato\u0159\u00EDch zavedeny, v kombinaci s cytometri\u00ED s vysok\u00FDm rozli\u0161en\u00EDm, techniky umo\u017E\u0148uj\u00EDc\u00ED sledov\u00E1n\u00ED \u017Eiv\u00FDch bun\u011Bk. Tyto techniky byly n\u00E1sledn\u011B pou\u017Eity pro studium vybran\u00FDch biologick\u00FDch syst\u00E9m\u016F. Rovn\u011B\u017E bylo nutn\u00E9 vyvino"@cs . . "1"^^ . "Neuvedeno."@en . . "http://www.isvav.cz/projectDetail.do?rowId=GA202/04/0907"^^ . . "27"^^ . . . "27"^^ . . "0"^^ . . "In the post-genomic era, the structure of the cell nucleus has become the key to understanding the most important cellular processes such as cell differentiation, apoptosis or transformation. In order to investigate highly organized nuclear structure in detail, methods of high-resolution cytometry developed in our laboratory in combination with techniques that enable visualization of nuclear structures in living cells will be used. DNA constructs coding fusion proteins will be employed. As model systemsTRF2, AIF, H1 and HP1 proteins will be conjugated to GFP and used to study their nuclear localization and dynamics in different cell lines. Interaction with DNA will be studied using subsequent 3D FISH in cells previously investigated in vivo. For both in vivo studies and FISH the technology of high-resolution cytometry will be used. Automated image acquisition, on-line analysis, storage and evaluation of the arrays of parameters in computer memory will be used. The main objective of the project will"@en . "In the frame of this project, we introduced experimental techniques of live cell observation and imaging into our laboratories, combined them with the high-resolution cytometry developed before and used in selected biological in vivo systems. Also algori"@en . . "Po rozlu\u0161t\u011Bn\u00ED genetick\u00E9ho k\u00F3du se struktura bun\u011B\u010Dn\u00E9ho j\u00E1dra stala kl\u00ED\u010Dovou pro pochopen\u00ED nejd\u016Fle\u017Eit\u011Bj\u0161\u00EDch bun\u011B\u010Dn\u00FDch proces\u016F jako jsou diferenciace, apopt\u00F3za a transformace. Abychom mohli detailn\u011B zkoumat vysoce organizovanou jadernou strukturu, pou\u017Eijememetodu cytometrie s vysok\u00FDm rozli\u0161en\u00EDm, kter\u00E1 byla vyvinuta v na\u0161\u00ED laborato\u0159i, sou\u010Dasn\u011B s technikami, je\u017E umo\u017E\u0148uj\u00ED vizualizaci jadern\u00FDch struktur v \u017Eiv\u00FDch bu\u0148k\u00E1ch. Jako modelov\u00E9 syst\u00E9my pou\u017Eijeme proteiny TRF2, AIF, H1 a HP1, kter\u00E9 p\u0159ipoj\u00EDme k GFP a budeme zkoumat u r\u016Fzn\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED jejich jadernou lokalizaci a dynamiku. Interakce s DNA bude zkoum\u00E1na n\u00E1slednou 3D FISH technikou v bu\u0148k\u00E1ch sledovan\u00FDch nejprve in vivo. Jak pro in vivo studie, tak pro studie na fixovan\u00FDch bu\u0148k\u00E1ch bude pou\u017Eita cytometrie s vysok\u00FDm rozli\u0161en\u00EDm, tj. automatizovan\u00E9 sn\u00EDm\u00E1n\u00ED obrazu, in-line anal\u00FDza, ukl\u00E1d\u00E1m na disk a vyhodnocov\u00E1n\u00ED soubor\u016F parametr\u016F v pam\u011Bti po\u010D\u00EDta\u010De. Hlavn\u00EDm c\u00EDlem projektu bude zaveden\u00ED GFP technologi\u00ED v na\u0161\u00ED laborato\u0159i a vyu\u017Eit\u00ED cytometrie s" . . . "Cytometrie s vysok\u00FDm rozli\u0161en\u00EDm na \u017Eiv\u00FDch bu\u0148k\u00E1ch" . "2007-10-16+02:00"^^ . . . . .