"1"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA202/02/1213"^^ . "Studovali jsme alosterick\u00E9 interakce na r\u016Fzn\u00FDch modelech nikotinick\u00E9ho acetylcholinov\u00E9ho receptoru exprimovan\u00E9ho v COS bu\u0148k\u00E1ch. Aktivaci a modulaci p\u0159irozen\u00FDch i mutovan\u00FDch nikotinick\u00FDch receptor\u016F (svalov\u00FD receptor embryon\u00E1ln\u00EDho i dosp\u011Bl\u00E9ho typu, neuron\u00E1"@cs . "Allosteric interactions were studied in a model of nicotinic acetylcholine receptors expressed in COS cells. Wildtype and mutated nicotinic receptor (mouse muscle embryonic and adult type, rat neuronal type alpha3beta4) activation was studied by the whol"@en . "The nicotinic acetylcholine receptor is a model ligand gated cationic channel which is allostericaly coupled with several interacting binding sites. Two agonist binding sites interact with classical agonists and competitive antagonists. At least two binding sites for noncompetitive agonists, antagonists and modulators influence the channel action. We will use a combination of biophysical methods (patch-clamp technique, microspectrofluorimetry and molecular biology) to investigate interaction of different binding sites of the nicotinic acetylcholine receptor under different conditions (temperature, cellular microenvironment, point mutations). Nicotinic receptors will be studied in the COS cell line expressing molecules after transfection with plasmids coding appropriate combination of natural or mutated receptor subunits. Specifically we will study the interactions involving intersubunit transfer of conformation changes - cooperativity and desensitization. Experimental results will be"@en . . "Interakce vazebn\u00FDch m\u00EDst nikotinick\u00E9ho acetylcholinov\u00E9ho receptoru" . . . . . . . . "2008-06-02+02:00"^^ . . . "0"^^ . . "Nikotinick\u00FD acetylcholinov\u00FD receptor je ligandem aktivovan\u00FD iontov\u00FD kan\u00E1l, \u0159\u00EDzen\u00FD n\u011Bkolika allostericky spojen\u00FDmi vazebn\u00FDmi m\u00EDsty. Dv\u011B vazebn\u00E1 m\u00EDsta interaguj\u00ED s\u00A0klasick\u00FDmi agonisty a kompetitivn\u00EDmi antagonisty. Nejm\u00E9n\u011B dv\u011B dal\u0161\u00ED vazebn\u00E1 m\u00EDsta interaguj\u00EDs\u00A0nekompetitivn\u00EDmi agonisty, antagonisty a modul\u00E1tory a ovliv\u0148uj\u00ED \u010Dinnost kan\u00E1lu.C\u00EDlem projektu je biofyzik\u00E1ln\u00EDmi metodami (metoda ter\u010D\u00EDkov\u00E9ho z\u00E1mku, fluorescen\u010Dn\u00ED mikroskopie, a molekul\u00E1rn\u00ED biologie) studovat interakce r\u016Fzn\u00FDch vazebn\u00FDch m\u00EDst nikotinick\u00E9ho acetylcholinov\u00E9ho receptoru za r\u016Fzn\u00FDch podm\u00EDnek (teplota, bun\u011B\u010Dn\u00E9 mikrookol\u00ED, specifick\u00E9 bodov\u00E9 mutace). Budeme studovat kan\u00E1ly exprimovan\u00E9 v\u00A0linii COS bun\u011Bk, kter\u00E1 vytv\u00E1\u0159\u00ED receptory po transfekci plasmidy k\u00F3duj\u00EDc\u00EDmi vhodn\u00E9 kombinace p\u0159irozen\u00FDch nebo mutovan\u00FDch podjednotek.Z\u00EDskan\u00E9 v\u00FDsledky budeme porovn\u00E1vat s\u00A0v\u00FDsledky, z\u00EDskan\u00FDmi na lini\u00EDch bun\u011Bk exprimuj\u00EDc\u00EDmi receptor p\u0159irozen\u011B. Zejm\u00E9na budeme studovat interakce, kter\u00E9 zahrnuj\u00ED p\u0159enos konforma\u010Dn\u00ED zm\u011Bny mezi jednotliv\u00FDmi" . . "17"^^ . . "17"^^ . . "Interaction of binding sites of nicotinic acetylcholine receptor"@en . "GA202/02/1213" . . "Neuvedeno."@en . . . . . . "0"^^ . .