"GA202/02/0804" . . . "Chronic myeloid leukemia radiation risk estimation based on BCR-ABL distances in hemotopoietic cells"@en . . . . "Ur\u010Den\u00ED radia\u010Dn\u00EDho rizika pro vznik chronick\u00E9 myeloidn\u00ED leuk\u00E9mie na z\u00E1klad\u011B m\u011B\u0159en\u00ED vzd\u00E1lenost\u00ED mezi geny ABL a BCR v hematopoietick\u00FDch bu\u0148k\u00E1ch" . "C\u00EDlem je vyu\u017E\u00EDt informace z\u00E1kladn\u00EDho v\u00FDzkumu pro odhad rizika indukce chronick\u00E9 myeloidn\u00ED leukemie ionizuj\u00EDc\u00EDm z\u00E1\u0159en\u00EDm. Ionizuj\u00EDc\u00ED z\u00E1\u0159en\u00ED je zn\u00E1m\u00FD karcinogen a jeho vliv na vznik chronick\u00E9 myeloidn\u00ED leukemie (CML) vypl\u00FDv\u00E1 z\u00A0\u010Detn\u00FDch studi\u00ED. CML je zp\u016Fsobena translokac\u00ED mezi geny ABL a BCR; t\u00E9m\u011B\u0159 v\u0161ichni pacienti maj\u00ED tuto translokaci a choroba je natolik roz\u0161\u00ED\u0159en\u00E1, \u017Ee jej\u00ED indukce z\u00E1\u0159en\u00EDm je jasn\u011B detekov\u00E1na v\u00A0epidemiologick\u00FDch studi\u00EDch. Pomoc\u00ED konfok\u00E1ln\u00ED mikroskopie budeme m\u011B\u0159it vzd\u00E1lenosti mezi geny BCR-ABL v\u00A0lidsk\u00FDch primitivn\u00EDch kmenov\u00FDch hematopoietick\u00FDch bu\u0148k\u00E1ch, v\u00A0nich\u017E pravd\u011Bpodobn\u011B doch\u00E1z\u00ED k\u00A0translokaci. Vzd\u00E1lenost mezi BCR a ABL geny men\u0161\u00ED ne\u017E 0.5 mikrometr\u016F jsme zjistili ve 3 - 20 % lymfocyt\u016F, co\u017E nazna\u010Duje, \u017Ee tyto geny jsou v bun\u011B\u010Dn\u00E9m\u00A0j\u00E1d\u0159e prostorov\u011B korelov\u00E1ny. Tato korelace m\u016F\u017Ee zv\u00FD\u0161it frekvenci BCR-ABL translokac\u00ED po oz\u00E1\u0159en\u00ED ionizuj\u00EDc\u00EDm z\u00E1\u0159en\u00EDm za p\u0159edpokladu, \u017Ee se vyskytuje tak\u00E9 u progenitorov\u00FDch bun\u011Bk. Data nam\u011B\u0159en\u00E1 v\u00A0t\u011Bchto bu\u0148k\u00E1ch budou p\u0159edstavovat nulovou d\u00E1vku" . . "2008-06-02+02:00"^^ . . . . "Our goal is to produce scientifically credible estimates of radiation-induced chronic myeloid leukemia (CML) risk. Ionizing radiation is a well-studied carcinogen known to cause CML and CML is well-undestood, homogenous and prevalent. CML is known to be caused by a translocation between ABL and BCR;almost all CML patients have the BCR-ABL translocation and CML induction is unequivocally detected in epidemiological studies. Using 3D confocal microscopy we shall measure BCR-to-ABL distances in human hematopoietic stem cells supposed to be the target cells of BCR-ABL translocation. BCR-to-ABL distance shorter than 0.5 micrometer was found in 3% to 20 % of human lymphocytes. Since this figure is much higher than expected for independent loci, BCR and ABL appear to be spatially correlated in some cells. This correlation can increase the frequency of BCR-ABL translocation if present also in target progenitor cells. Data obtained in these cells will represent zero-dose data and will be relevant to CML"@en . . "13"^^ . "Neuvedeno."@en . . "13"^^ . . . "0"^^ . . . . "Our results show that irradiation of human hematopoietic progenitor cells by the dose of 2 Gy raises the fraction of cells with close proximity (L 0.4 u) of ABL and BCR genes. By this, the probability of the induction of translocation between these genes"@en . "http://www.isvav.cz/projectDetail.do?rowId=GA202/02/0804"^^ . . . . "1"^^ . "Na\u0161e v\u00FDsledky ukazuj\u00ED, \u017Ee po oz\u00E1\u0159en\u00ED lidsk\u00FDch hematopoietick\u00FDch kmenov\u00FDch bun\u011Bk d\u00E1vkou 2 Gy doch\u00E1z\u00ED ke zv\u00FD\u0161en\u00ED frakce bun\u011Bk, v nich\u017E jsou geny ABL a BCR vz\u00E1jemn\u011B bl\u00ED\u017Ee ne\u017E 0.4 um, co\u017E zvy\u0161uje pravd\u011Bpodobnost vzniku translokac\u00ED mezi t\u011Bmito geny a t\u00EDm i ch"@cs . . "1"^^ .