. "Grant p\u0159ed\u010Dasn\u011B ukon\u010Den na \u017E\u00E1dost \u0159e\u0161itele. \u00DAdaje uveden\u00E9 v z\u00E1v\u011Bre\u010Dn\u00E9 kart\u011B jsou adekv\u00E1tn\u00ED. Publika\u010Dn\u00ED \u010Dinnost je v\u00FDborn\u00E1. Nedostatky v \u010Derp\u00E1n\u00ED finan\u010Dn\u00EDch prost\u0159edk\u016F nejsou."@cs . "Specific interactions of new porphyrin analogues with nucleic acids"@en . "0"^^ . . "1"^^ . "GA202/00/P069" . "0"^^ . . "The aim of this project is spectroscopic study of interaction of tetraphenyl- and tetrapyridyl- porphyrin derivatives containing large charged groups with polynucleotides and DNA. Using UV-Vis absorption spectroscopy, resonance Raman scattering and surface-enhanced resonance Raman scattering spectroscopies formation, structure and stability of these complexes in very low concentrations will be studied. The main goal of this project is determine how the new porphyrin derivatives can interact with nucleic acids, how this interaction depends on the porphyrin self-aggregation, what is the structure and stability of porphyrin/nucleic acid complexes and what is the influence of anions stabilizing nucleic acids, of sequences of the base pairs and of porphyrin structure itself on geometry of the complex. Results obtained during this study allow us to preselect new promising porphyrin modifications, that could serve as a efficient photosenzibilizators in photodynamic therapy of cancer. These selected"@en . "1"^^ . . "1"^^ . . . . "http://www.isvav.cz/projectDetail.do?rowId=GA202/00/P069"^^ . "N\u00E1pln\u00ED projektu je spektroskopick\u00E9 studium interakc\u00ED vybran\u00FDch deriv\u00E1t\u016F tetrafenyl- a tetrapyridyl- porfyrin\u016F obsahuj\u00EDc\u00EDch v\u00EDceatomov\u00E9 nabit\u00E9 postrann\u00ED skupiny s polynukleotidy a p\u0159irozenou DNA. Pomoc\u00ED UV-Vis absorp\u010Dn\u00ED spektroskopie a spektroskopi\u00ED resonan\u010Dn\u00EDho Ramanova rozptylu a povrchem zes\u00EDlen\u00E9ho resonan\u010Dn\u00EDho Ramanova rozptylu bude sledov\u00E1na tvorba, struktura a stabilita t\u011Bchto komplex\u016F za velmi n\u00EDzk\u00FDch koncentrac\u00ED. Hlavn\u00EDm c\u00EDlem projektu je ur\u010Dit, do jak\u00E9 m\u00EDry mohou nov\u00E9 deriv\u00E1ty porfyrin\u016F interagovat s nukleov\u00FDmi kyselinami, jak z\u00E1vis\u00ED tato intrakce na stupni agregace porfyrin\u016F, jak\u00E9 je uspo\u0159\u00E1d\u00E1n\u00ED a stabilita komplex\u016F porfyrin/nukleov\u00E1 kyselina a jak\u00FD je vliv aniont\u016F stabilizuj\u00EDc\u00ED strukturu nukleov\u00FDch kyselin, sekvence b\u00E1zov\u00FDch p\u00E1r\u016F, p\u0159\u00EDpadn\u011B porfyrinov\u00E9 struktury na geometrii komplex\u016F. Z\u00EDskan\u00E9 informace pomohou vytipovat nov\u00E9 nad\u011Bjn\u00E9 modifikace porfyrin\u016F, kter\u00E9 by mohly slou\u017Eit jako \u00FA\u010Dinn\u00E9 fotosenzibiliz\u00E1tory p\u0159i fotodynamick\u00E9 terapii rakoviny. Takto vybran\u00E9 l\u00E1tky pak budou d\u00E1le stu" . "Specifick\u00E1 interakce nov\u00FDch analog porfyrin\u016F s nukleov\u00FDmi kyselinami" . . . . . . . . .