. "GA14-32432S" . . . "0"^^ . . "Linking microbial meta-omics with ecosystem functioning: populations and pathways involved in chloroethenes degradation"@en . . . "Chlorovan\u00E9 etyleny (CE) byly v 20. stolet\u00ED hojn\u011B vyu\u017E\u00EDv\u00E1ny jako \u00FA\u010Dinn\u00E1 neho\u0159lav\u00E1 rozpou\u0161t\u011Bdla, a proto nyn\u00ED pat\u0159\u00ED mezi nej\u010Dast\u011Bj\u0161\u00ED persistentn\u00ED polutanty v p\u016Fd\u00E1ch a podzemn\u00EDch vod\u00E1ch. P\u0159i degradaci CE doch\u00E1z\u00ED \u010Dasto k akumulaci toxi\u010Dt\u011Bj\u0161\u00EDch meziprodukt\u016F, p\u0159i\u010Dem\u017E mechanismus jejich vzniku i odbour\u00E1v\u00E1n\u00ED nen\u00ED st\u00E1le uspokojiv\u011B objasn\u011Bn. Na\u0161e studie propojuje informace o indigenn\u00EDch mikrobi\u00E1ln\u00EDch populac\u00EDch a jejich funk\u010Dn\u00EDch genech s metabolickou aktivitou b\u011Bhem degradace CE. Modern\u00ED vysokokapacitn\u00ED sekvena\u010Dn\u00ED metody v kombinaci s metodami funk\u010Dn\u00ED mikrobi\u00E1ln\u00ED ekologie (nap\u0159. stable isotope probing, SIP) umo\u017En\u00ED nov\u00FD n\u00E1hled do t\u011Bchto dosud m\u00E1lo prozkouman\u00FDch mechanism\u016F a vztah\u016F. SIP selektuje tu \u010D\u00E1st mikrobi\u00E1ln\u00EDho spole\u010Denstva, kter\u00E1 se p\u0159\u00EDmo pod\u00EDl\u00ED na asimilaci CE, meta\u2013omika umo\u017En\u00ED detailn\u00ED vhled do fungov\u00E1n\u00ED spole\u010Denstva s mo\u017Enost\u00ED sledov\u00E1n\u00ED nejen fylogenetick\u00E9 diversity (metagenomika), ale i funk\u010Dn\u00EDho rozli\u0161en\u00ED (metatranskriptomika, metaproteomika). V\u00FDsledkem projektu bude zmapov\u00E1n\u00ED metabolick\u00FDch drah a aktivn\u00EDch gen\u016F zapojen\u00FDch do degradace CE v re\u00E1ln\u00FDch podm\u00EDnk\u00E1ch \u017Eivotn\u00EDho prost\u0159ed\u00ED." . . . . . "http://www.isvav.cz/projectDetail.do?rowId=GA14-32432S"^^ . . "Chlorinated ethenes (CE) have been used as effective non-flammable solvents. Therefore, they are widespread persistent pollutants of soils and waters. Degradation of CE often results in the formation of more toxic compounds. Mechanism of their formation and degradation still remains rather unclear. This study aims to link specific microorganisms, genes, and metabolites to in situ CE degradation activities. Modern high throughput sequencing methods along with techniques of functional microbial ecology (e.g., stable isotope probing, SIP) enable new insights into the complexity of such mechanisms. SIP will select the populations directly involved in the assimilation of CE, microbial meta-omics will clarify the functionality of the communities, including phylogenetic diversity (metagenomics) and metabolic diversity (metatranscriptomics, metaproteomics). The outputs of the project will include metabolic pathways mapping and identification of genes involved in the degradation of CE in real environmental conditions."@en . . . . " metagenomics" . "2015-04-23+02:00"^^ . . "2016-12-31+01:00"^^ . " biodegradation" . "2014-03-31+02:00"^^ . . "chloroethenes, biodegradation, biotransformation, metagenomics, metatranscriptomics"@en . . "2014-01-01+01:00"^^ . "Mikrobi\u00E1ln\u00ED meta-omika v souvislosti s fungov\u00E1n\u00EDm ekosyst\u00E9m\u016F: role populac\u00ED a jejich metabolick\u00FDch drah v degradaci chlorethen\u016F" . "1"^^ . "chloroethenes" . "0"^^ . "1"^^ . "0"^^ . " biotransformation" .