. "N-methyl-D-aspart\u00E1tov\u00E9 (NMDA) receptory zprost\u0159edkov\u00E1vaj\u00ED glutam\u00E1tergn\u00ED synaptick\u00FD p\u0159enos v centr\u00E1ln\u00EDm nervov\u00E9m syst\u00E9mu savc\u016F. Abnorm\u00E1ln\u00ED regulace NMDA receptor\u016F je spojov\u00E1na se vznikem mnoha neurologick\u00FDch a psychiatrick\u00FDch onemocn\u011Bn\u00ED. P\u0159esto\u017Ee je zn\u00E1mo v\u00EDce ne\u017E 10 let, \u017Ee NMDA receptory jsou N-glykosylov\u00E1ny, nen\u00ED jasn\u00E9, jakou roli tato modifikace hraje p\u0159i regulaci jejich funkce v neuronech. C\u00EDlem tohoto projektu je objasnit, pomoc\u00ED multidisciplin\u00E1rn\u00EDho p\u0159\u00EDstupu zahrnuj\u00EDc\u00EDho molekul\u00E1rn\u00ED biologii, biochemii, imunocytochemii a elektrofyziologii, i) jak\u00E1 N-glykosyla\u010Dn\u00ED m\u00EDsta v GluN1 a GluN2 podjednotk\u00E1ch a/nebo kter\u00E9 typy N-glykan\u016F jsou kritick\u00E9 pro transport NMDA receptor\u016F na bun\u011B\u010Dn\u00FD povrch a ii) zda-li N-glykosylace reguluje funk\u010Dn\u00ED vlastnosti NMDA receptor\u016F. Tento projekt p\u0159isp\u011Bje k porozum\u011Bn\u00ED regula\u010Dn\u00EDch mechanizm\u016F mozkov\u00FDch funkc\u00ED." . . . . . . . . "Regulation of NMDA receptor trafficking and function by N-glycosylation"@en . "N-methyl-D-aspartate (NMDA) receptors play critical roles in glutamatergic neurotransmission at excitatory synapses in the mammalian brain. It is well-established that the abnormal regulation of NMDA receptors underlies a wide variety of neurological and psychiatric disorders. Although it has been known for more than a decade that NMDA receptors are N-glycosylated, the effect of this modification on the regulation of NMDA receptors in complex cells such as neurones remains poorly understood. In this project, we will combine the state-of-the-art methodologies of two different disciplines\u2014neurobiology and glycobiology\u2014with molecular biology, biochemistry, immunocytochemistry, electrophysiology to elucidate i) if the N-glycosylation motifs within the GluN1 and GluN2 subunits and/or specific types of N-glycans are critical for surface delivery of NMDA receptors and ii) whether N-glycosylation regulates the functional properties of NMDA receptors. The acquisition of such knowledge is critical to our understanding of the molecular mechanisms underlying the regulation of brain function."@en . " glutamate" . " ion channel" . "2014-01-01+01:00"^^ . " endoplasmic reticulum" . "GA14-02219S" . . . "2015-04-23+02:00"^^ . . . . . " trafficking" . . "Regulace transportu a funkce NMDA receptor\u016F N-glykosylac\u00ED" . "2014-03-14+01:00"^^ . "1"^^ . "NMDA receptor; ion channel; trafficking; endoplasmic reticulum; glutamate; subunit assembly."@en . . "http://www.isvav.cz/projectDetail.do?rowId=GA14-02219S"^^ . "0"^^ . "2"^^ . "2"^^ . "NMDA receptor" . "2016-12-31+01:00"^^ . "0"^^ . . .