" Juncaceae" . . "http://www.isvav.cz/projectDetail.do?rowId=GA13-29362S"^^ . " holokinetic drive" . . . "2013-02-01+01:00"^^ . "Adaptivn\u00ED vznik holocentrick\u00FDch chromosom\u016F (bez lokalizovan\u00E9 centromery) jako obrany proti centromerick\u00E9mu tahu bude testov\u00E1n pomoc\u00ED selek\u010Dn\u00EDch re\u017Eim\u016F p\u016Fsob\u00EDc\u00EDch na geny kinetochorov\u00FDch protein\u016F. K objasn\u011Bn\u00ED p\u0159ekvapiv\u011B negativn\u00ED korelace 2C/2n a zna\u010Dn\u00E9 divergence velikost\u00ED a po\u010Dt\u016F holocentrick\u00FDch chromosom\u016F je navr\u017Een nov\u00FD evolu\u010Dn\u00ED mechanismus %22holokinetick\u00FD tah%22, kter\u00FD bude testov\u00E1n anal\u00FDzou segregace fragmentovan\u00FDch/nefragmentovan\u00FDch homolog\u016F v potomstvu struktur\u00E1ln\u00EDch heterozygot\u016F p\u0159ipraven\u00FDch rentgenov\u00FDm z\u00E1\u0159en\u00EDm. Velikost genomu a genomick\u00FD obsah GC, proporce a efektivita jednotliv\u00FDch mechanism\u016F karyotypov\u00E9 evoluce (aneuploidie, polyploidie, f\u00FAze/fragmentace chromosom\u016F, proliferace/odstra\u0148ov\u00E1n\u00ED repetitivn\u00ED DNA) budou analyzov\u00E1ny v holocentrick\u00FDch (Cyperaceae+Juncaceae, Chionographideae, Cuscuta subg. Cuscuta a Grammica, Drosera) a jejich sestersk\u00FDch monocentrick\u00FDch lini\u00EDch pomoc\u00ED pr\u016Ftokov\u00E9 cytometrie, po\u010D\u00EDt\u00E1n\u00ED chromosom\u016F a nov\u011B vyvinut\u00E9ho fylogentick\u00E9ho algoritmu. Bude vyvinuta nov\u00E1 detek\u010Dn\u00ED technika pro mas\u00EDvn\u00ED skr\u00EDning holocentrismu u rostlin kombinuj\u00EDc\u00ED z\u00E1\u0159en\u00ED a cytometrii." . . "centromere drive, chromosomal fusion/fission, Cyperaceae, flow cytometry, genome size, genomic GC content, holocentric chromosomes, holokinetic drive, Juncaceae, karyotype, meiotic drive"@en . " flow cytometry" . "An adaptive origin of holocentric chromosomes (without localized centromere) as a defense against centromere drive will be analyzed using selection regimes acting on kinetochore proteins. To explain surprisingly negative 2C/2n correlation and extreme divergences in holocentric chromosome sizes and numbers we propose a new evolutionary mechanism of \u201Cholokinetic drive\u201D that will be tested by the study of segregation ratio of fragmented/non-fragmented homologs in the progeny of structural heterozygotes prepared via x-irradiation. Genome size, genomic GC%, portions and rates of particular modes of karyotype evolution (aneuploidy, polyploidy, chromosomal fusion/fission and repetitive DNA proliferation/removal) will be analyzed in holocentrics (Cyperaceae+Juncaceae, Chionographideae, Cuscuta subg. Cuscuta and Grammica, Drosera) and their sister monocentric clades using flow cytometry, chromosome counting and newly developed phylogenetic algorithm. A new detection technique combining irradiation and flow cytometry will be developed for an effective screening for holocentrism in plants."@en . "Evoluce holocentrick\u00FDch chromosom\u016F" . "0"^^ . . . "1"^^ . "Evolution of holocentric chromosomes"@en . "2015-04-23+02:00"^^ . " karyotype" . . "3"^^ . "0"^^ . "3"^^ . . . " chromosomal fusion/fission" . . " genome size" . . " genomic GC content" . " holocentric chromosomes" . . "2014-03-19+01:00"^^ . "2016-12-31+01:00"^^ . "GA13-29362S" . . . . "centromere drive" . " Cyperaceae" . . .