"Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development" . "7"^^ . . " hormonal metabolism" . "0"^^ . "1"^^ . . "hormonal regulation of plant development, cytokinins, ethylene, auxin transport, hormonal metabolism, signaling, multistep phosphorelay, structure and function of sensor histidine kinases, Arabidopsis thaliana"@en . "2017-12-31+01:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA13-25280S"^^ . . . "0"^^ . " auxin transport" . " cytokinins" . "2014-04-18+02:00"^^ . . "Cytokininy (CK) a etyl\u00E9n jsou d\u016Fle\u017Eit\u00FDmi regul\u00E1tory v\u00FDvoje rostlin a jejich p\u016Fsoben\u00ED spolu \u00FAzce souvis\u00ED. Na\u0161e nepublikovan\u00E9 v\u00FDsledky ukazuj\u00ED na dosud nezn\u00E1mou roli CK v indukci n\u011Bkolika protein\u016F pod\u00EDlej\u00EDc\u00EDch se na biosynt\u00E9ze etyl\u00E9nu. D\u00E1le jsme identifikovali interferenci CK a etyl\u00E9nov\u00E9ho sign\u00E1lu v syst\u00E9mu v\u00EDcestup\u0148ov\u00E9ho p\u0159enosu fosf\u00E1tu (MSP). C\u00EDlem projektu je objasn\u011Bn\u00ED \u010Dasoprostorov\u00E9 specifity a v\u00FDvojov\u00E9ho v\u00FDznamu interakce CK a etyl\u00E9nu na \u00FArovni regulace nov\u011B identifikovan\u00FDch protein\u016F pod\u00EDlej\u00EDcich se na synt\u00E9ze etyl\u00E9nu a molekul\u00E1rn\u00EDch mechanism\u016F intereference sign\u00E1lu CK a etyl\u00E9nu v MSP. Kone\u010Dn\u011B pl\u00E1nujeme objasnit podstatu molekul\u00E1rn\u00EDho p\u0159ep\u00EDna\u010De, jeho\u017E p\u0159\u00EDtomnost n\u00E1\u0161 model p\u0159edpokl\u00E1d\u00E1 a kter\u00FD pravd\u011Bpodobn\u011B p\u0159esm\u011Brov\u00E1v\u00E1 p\u0159enos etyl\u00E9nov\u00E9ho sign\u00E1lu mezi z\u00E1kladn\u00ED sign\u00E1ln\u00ED kask\u00E1dou zprot\u0159edkovanou MAP kin\u00E1zami a MSP. Za t\u00EDmto \u00FA\u010Delem budeme pomoc\u00ED NMR a rentgenov\u00E9 krystalografie analyzovat strukturn\u00ED zm\u011Bny histidinkin\u00E1zov\u00FDch dom\u00E9n ethyl\u00E9nov\u00FDch receptor\u016F ETR1 a/nebo ERS1 v p\u0159\u00EDtomnosti r\u016Fzn\u00FDch divalentn\u00EDch iont\u016F, kter\u00E9 byly identifikov\u00E1ny jako regul\u00E1tory jejich substr\u00E1tov\u00E9 specifity." . " multistep phosphorelay" . . "2015-04-23+02:00"^^ . . . . " ethylene" . "Objasn\u011Bn\u00ED molekul\u00E1rn\u00EDch mechanizm\u016F interakce cytokinin\u016F a etyl\u00E9nu ve v\u00FDvoji rostlin"@en . . " signaling" . "hormonal regulation of plant development" . " structure and function of sensor histidine kinases" . . "Cytokinins (CK) and ethylene are important regulators of plant development that are tightly interconnected in their action. Our unpublished results show previously unknown upregulation of several proteins from the ethylene biosynthetic pathway by CK. Further, we recently identified interference of CK and ethylene signaling in the multistep phosphorelay (MSP) pathway. The goal of the proposed project is elucidating the spatiotemporal specificity and developmental consequences of CK-ethylene crosstalk at the level of newly identified upregulation of ethylene biosynthetic proteins by CK and molecular mechanisms of CK and ethylene interference in the MSP signaling. Finally, we intend to identify the nature of molecular switch proposed in our model that might redirect ethylene signaling from its canonical MAP kinase cascade to the MSP. To do that, we will employ NMR and X-ray crystallography to analyze structural changes of histidine kinase domains of ethylene receptor ETR1 and/or ERS1 in the presence of diverse divalent cations that were shown to modify their substrate specificity."@en . . "2013-02-01+01:00"^^ . . . "GA13-25280S" . . . . "7"^^ .