" whole mount confocal imaging" . . . "http://www.isvav.cz/projectDetail.do?rowId=GA13-12412S"^^ . . . . "2014-04-18+02:00"^^ . . . "2017-12-31+01:00"^^ . . . "2015-04-23+02:00"^^ . "GA13-12412S" . "Development of mouse conduction system analyzed by 3D imaging and optical mapping"@en . "7"^^ . . "7"^^ . " CLSM" . . . "1"^^ . "mouse embryo, OPT, whole mount confocal imaging, CLSM, TPEM, GFP, arrhythmias"@en . "1"^^ . " GFP" . . "mouse embryo" . " TPEM" . "V\u00FDvoj p\u0159evodn\u00EDho syst\u00E9mu u my\u0161i analyzovan\u00FD 3D zobrazovac\u00EDmi metodami a optick\u00FDm mapov\u00E1n\u00EDm" . "0"^^ . "2013-02-01+01:00"^^ . . . . "Cardiac conduction system (CCS) is a specialized network of tissues responsible for generation and coordinated spread of excitation through the heart. Despite recent advances in its characterization on morphological, functional and molecular level, the mechanisms of connection of different components specified during development through distinct mechanisms are poorly understood. Our aims are to develop new multi-dimensional imaging modalities to investigate the morphological and functional architecture of this network during mouse development. Experiments will be performed in vivo to address the question how the ventricular CS is connected proximally to the atrioventricular node and distally to the working myocardium. We will use transgenic mouse lines to visualize the connexin40-expressing components of the CCS in three dimensions using eGFP and combine this information with data showing impulse propagation through the heart. Together, this data will enhance our understanding of normal CCS development as well as provide explanation of developmental mechanisms of arrhythmias."@en . . " OPT" . "P\u0159evodn\u00ED syst\u00E9m srde\u010Dn\u00ED je s\u00ED\u0165 specializovan\u00FDch tk\u00E1n\u00ED odpov\u011Bdn\u00FDch za vznik a koordinovan\u00E9 \u0161\u00ED\u0159en\u00ED elektrick\u00E9ho impulsu v srdci. Navzdory pokroku v jeho charakterizaci na morfologick\u00E9, funk\u010Dn\u00ED i molekul\u00E1rn\u00ED \u00FArovni jsou mechanizmy propojen\u00ED jeho komponent, specifikovan\u00FDch za v\u00FDvoje specifick\u00FDmi molekul\u00E1rn\u00EDmi mechanizmy nejasn\u00E9. Na\u0161e c\u00EDle spo\u010D\u00EDvaj\u00ED ve v\u00FDvoji nov\u00E9 multimod\u00E1ln\u00ED trojrozm\u011Brn\u00E9 zobrazovac\u00ED metody schopn\u00E9 zachytit tyto zm\u011Bny architektury b\u011Bhem v\u00FDvoje my\u0161\u00EDho z\u00E1rodku. Provedeme in vivo pokusy s c\u00EDlem charakterizovat spojen\u00ED komorov\u00E9ho p\u0159evodn\u00EDho syst\u00E9mu proxim\u00E1ln\u011B se s\u00ED\u0148okomorov\u00FDm uzlem a dist\u00E1ln\u011B s pracovn\u00EDm myokardem. Vyu\u017Eijeme transgenn\u00EDch my\u0161\u00EDch lini\u00ED s expres\u00ED zelen\u00E9ho fluorescen\u010Dn\u00EDho proteinu v p\u0159evodn\u00EDm syst\u00E9mu, a tyto \u00FAdaje zkombinujeme s daty z\u00EDskan\u00FDmi technikou optick\u00E9ho mapov\u00E1n\u00ED, je\u017E ukazuje \u0161\u00ED\u0159en\u00ED vzruchu v srdci. Tato data prohloub\u00ED na\u0161e pozn\u00E1n\u00ED v\u00FDvoje p\u0159evodn\u00EDho syst\u00E9mu a zlep\u0161\u00ED ch\u00E1pan\u00ED v\u00FDvojov\u00E9ho podkladu vzniku poruch srde\u010Dn\u00EDho rytmu." . .