. . "2013-02-01+01:00"^^ . "oocyte" . " RNA" . " meiosis" . . "Mammalian oocyte is highly differentiated totipotent cell, which gives foundation to embryo development. Importantly, fully grown germinal vesicle oocyte is transcriptionally inactive, and utilizes only transcripts synthesized and stored during earlier development. Mechanisms of mRNA metabolism in the mammalian oocyte are still far from being fully understood. We will test our hypothesis that mammalian GV oocyte mRNA asymmetry is defined by nuclear retention of specific mRNAs and this localization is essential for the generation of fertilizable oocyte. Thus, the objective of the current project is transcriptome profiling of the nucleus from fully grown mouse oocyte by sequencing. Furthermore, we aim to detect localization of identified mRNAs, mechanism of nuclear-retention and to understand physiological relevance of their retention. This represents a first attempt to produce a unique resource of nuclear-retained transcripts and characterization of mRNA metabolism in order to understand molecular physiology of mammalian oocyte."@en . "2017-12-31+01:00"^^ . . . "1"^^ . . "0"^^ . "1"^^ . "Retention of specific mRNAs in the nucleus of the fully grown mammalian oocyte"@en . . "1"^^ . . "2015-04-23+02:00"^^ . . . . . . "Sav\u010D\u00ED oocyt je vysoce diferencovan\u00E1 totipotentn\u00ED bu\u0148ka, kter\u00E1 d\u00E1v\u00E1 z\u00E1klad embryon\u00E1ln\u00EDmu v\u00FDvoji. Oocyt s dokon\u010Den\u00FDm r\u016Fstem p\u0159ed zah\u00E1jen\u00EDm mei\u00F3zy postr\u00E1d\u00E1 transkrip\u010Dn\u00ED aktivitu a vyu\u017E\u00EDv\u00E1 pouze matern\u00E1ln\u00ED mRNA, kter\u00E9 byly nasyntetizov\u00E1ny b\u011Bhem jeho v\u00FDvoje. Mechanismy regulace matern\u00E1ln\u00ED mRNA jsou z velk\u00E9 \u010D\u00E1sti nezn\u00E1m\u00E9. Z\u00E1m\u011Brem projektu je zmapovat RNA lokalizovan\u00E9 v j\u00E1d\u0159e pln\u011B vzrostl\u00E9ho my\u0161\u00EDho oocytu ve st\u00E1diu GV (germinal vesicle-z\u00E1rode\u010Dn\u00FD v\u00E1\u010Dek) pomoc\u00ED sekvenace. Chceme tak\u00E9 identifikovat lokalizaci specifick\u00FDch mRNA v my\u0161\u00EDm, prase\u010D\u00EDm a lidsk\u00E9m oocytu a objasnit mechanismus jejich retence v j\u00E1d\u0159e. D\u00E1le je projekt zam\u011B\u0159en na lokalizovanou translaci v oocytu a na synt\u00E9zu specifick\u00FDch protein\u016F bezprost\u0159edn\u011B po zah\u00E1jen\u00ED mei\u00F3zy. Jedn\u00E1 se o prvn\u00ED snahu identifikovat jadern\u00FD transkriptom s c\u00EDlem charakterizovat metabolismus specifick\u00FDch RNA a p\u0159isp\u011Bt tak k pozn\u00E1n\u00ED fyziologie sav\u010D\u00EDho oocytu." . "0"^^ . " translation" . . "http://www.isvav.cz/projectDetail.do?rowId=GA13-12291S"^^ . . "2014-03-19+01:00"^^ . "Retence specifick\u00FDch mRNA v j\u00E1d\u0159e sav\u010D\u00EDho oocyt\u016F" . "GA13-12291S" . . "oocyte, RNA, translation, meiosis, nucleus"@en . .