"The use of biofuels in vehicles introduces the need for better understanding of the biological impacts of exposure to particulate matter emitted by the engines, a major urban health issue. The project will use the model of human lung cells (HEL, A549) to partly solve this problem. Respirable particles (PM2.5) from engine emissions produced by selected commonly used biofuels and, for comparison, by classic diesel fuel and gasoline, will be collected from various engines during various test cycles. The chemical analysis of extracts from sampled PM2.5 will be performed and selected toxicity markers will be analyzed in cell cultures. Genome-wide transcriptomics and selected protein expression will be employed to get more insight into the mechanisms of biological efects of engine emissions. Combined use of chemical analysis with genomics will enable to identify major toxic emission components as well as biological pathways involved. Project will contribute substantially to direct comparison of mechanisms of the toxicity of biofuels with classic fuels and may be used for a risk assessment."@en . "2015-12-31+01:00"^^ . "0"^^ . . . "P\u016Fsoben\u00ED emis\u00ED z dopravy na \u010Dlov\u011Bka zahrnuje \u0159adu biologick\u00FDch proces\u016F. Pou\u017E\u00EDv\u00E1n\u00ED biopaliv vytvo\u0159ilo aktu\u00E1ln\u00ED probl\u00E9m hlub\u0161\u00EDho a komplexn\u00EDho porozumn\u011Bn\u00ED biologick\u00FDm mechanism\u016Fm p\u016Fsoben\u00ED automobilov\u00FDch emis\u00ED p\u0159i pou\u017Eit\u00ED biopaliv. Projekt se zam\u011B\u0159uje na charakterizaci slo\u017Een\u00ED sm\u011Bs\u00ED v\u00E1zan\u00FDch na respirabiln\u00ED \u010D\u00E1stice (PM2.5) a jejich toxick\u00FDch \u00FA\u010Dink\u016F v modelech lidsk\u00FDch plicn\u00EDch bun\u011Bk. Budou odebr\u00E1ny respirabiln\u00ED \u010D\u00E1stice z emis\u00ED z vybran\u00FDch biopaliv a pro porovn\u00E1n\u00ED z klasick\u00E9 nafty a benz\u00EDnu s pou\u017Eit\u00EDm r\u016Fzn\u00FDch typ\u016F motor\u016F a testovac\u00EDch re\u017Eim\u016F. U extrakt\u016F z odebran\u00FDch PM2.5 bude provedena chemick\u00E1 anal\u00FDza a budou stanoveny markery toxicity v plicn\u00EDch bun\u011B\u010Dn\u00FDch kultur\u00E1ch (HEL, A549). Pro anal\u00FDzu mechanism\u016F p\u016Fsoben\u00ED emis\u00ED bude sledov\u00E1na genov\u00E1 exprese na celogenomov\u00E9 \u00FArovni i exprese vybran\u00FDch protein\u016F. Sou\u010Dasn\u00E9 pou\u017Eit\u00ED chemick\u00E9 anal\u00FDzy kl\u00ED\u010Dov\u00FDch slo\u017Eek emis\u00ED a toxikogenomiky povede k identifikaci hlavn\u00EDch toxick\u00FDch slo\u017Eek emis\u00ED a z\u00FA\u010Dastn\u011Bn\u00FDch biologick\u00FDch proces\u016F. V\u00FDsledky umo\u017En\u00ED srovn\u00E1n\u00ED mechanism\u016F toxicity biopaliv a klasick\u00FDch paliv a mohou b\u00FDt vyu\u017Eity pro hodnocen\u00ED zdravotn\u00EDch rizik." . . "1"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=GA13-01438S"^^ . "2"^^ . "2013-02-01+01:00"^^ . . . "air pollution" . . "Mechanismy toxicity pevn\u00FDch emis\u00ED z biopaliv" . . . "Mechanisms of toxicity of biofuel particulate emissions"@en . " biofuels" . "air pollution, biofuels, vehicle emissions, toxicogenomics"@en . . "10"^^ . . "10"^^ . "2015-04-23+02:00"^^ . "2014-04-18+02:00"^^ . . . " vehicle emissions" . . "GA13-01438S" . . . . .