"Hlavn\u00EDm c\u00EDlem tohoto projektu bude z\u00EDskat nov\u00E9 z\u00E1kladn\u00ED poznatky diagnosticky zaj\u00EDmav\u00FDch molekul a d\u00E1le pak metodick\u00E9 znalosti umo\u017E\u0148uj\u00EDc\u00ED v\u00FDvoj a pou\u017Eit\u00ED imunochemick\u00FDch metod pro detekci patogenn\u00EDch bakteri\u00ED, v na\u0161em p\u0159\u00EDpad\u011B F. tularensis, kter\u00E9 p\u0159ekonaj\u00ED nedostatky aktu\u00E1ln\u011B pou\u017E\u00EDvan\u00FDch diagnostick\u00FDch p\u0159\u00EDstup\u016F. K dosa\u017Een\u00ED tohoto c\u00EDle budeme prov\u00E1d\u011Bt ve vyv\u00E1\u017Een\u00E9 m\u00ED\u0159e z\u00E1kladn\u00ED, aplikovan\u00FD a experiment\u00E1ln\u00ED v\u00FDzkum metod sm\u011B\u0159uj\u00EDc\u00EDch k nalezen\u00ED nov\u00FDch kandid\u00E1tn\u00EDch molekul pro typizaci mikroba a k z\u00EDsk\u00E1n\u00ED unik\u00E1tn\u00EDho souboru antigen\u016F a protil\u00E1tek s vysokou specifitou, co\u017E povede k vylep\u0161en\u00ED dosavadn\u00EDch serologick\u00FDch test\u016F." . "2009-07-01+02:00"^^ . . . . . . "2014-07-17+02:00"^^ . "molecular diagnostic" . . "molecular diagnostic; typing; mass spectrometry; SILAC; Francisella tularensis"@en . . . "2"^^ . . "2"^^ . "http://www.isvav.cz/projectDetail.do?rowId=FR-TI1/292"^^ . "1"^^ . " mass spectrometry" . "2"^^ . . "The set of recombinant membrane proteins and specific polyclonal rabbit antiodies has been prepared. They will be used for the selective immune-detection and diagnosis of subtypes of two differently virulent bacterial strains of Francisella tularensis."@en . "0"^^ . " typing" . "Molecular diagnostic of bacterial pathogens"@en . "Molekul\u00E1rn\u00ED diagnostika bakteri\u00E1ln\u00EDch patogen\u016F" . . "The main goal of the proposal is to create new unbiased and fully reliabledetection system for pathogenic bacteria, e.g. F. tularensis. Basicresearch, aimed at the identification of diagnostic protein markers, willbe followed by experimental and applied research which will targetrecombinant forms of protein markers and production of highly specificantibodies. Finally the overall methodology and protein and antibodyproducts will be utilized in creation of novel detection systems."@en . "2013-06-30+02:00"^^ . " SILAC" . "2013-05-10+02:00"^^ . . . . . . . . "Byl z\u00EDsk\u00E1n soubor rekombinantn\u00EDch membr\u00E1nov\u00FDch protein\u016F a specifick\u00FDch polyklon\u00E1ln\u00EDch kr\u00E1li\u010D\u00EDch protil\u00E1tek, kter\u00E9 budou vyu\u017Eity pro selektivn\u00ED imuno-detekci a subtypovou diagnostiku dvou r\u016Fzn\u011B virulentn\u00EDch bakt. Kmen\u016F Francisella tulearensis."@cs . . "FR-TI1/292" .