"2014-11-30+01:00"^^ . . . "This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes\u2019 specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution." . . "Glycoside; transglycosidase; phosphorylase; directed evolution; metagenome; fluorescence probes"@en . . . " phosphorylase" . . . "Glycoside" . " metagenome" . . . " directed evolution" . . "This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes\u2019 specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution."@en . . " transglycosidase" . "2015-02-16+01:00"^^ . . "13"^^ . "13"^^ . . . "7E11011" . "2013-03-27+01:00"^^ . . . "2011-01-01+01:00"^^ . . "Novel Biocatalysts for the Production of Glycosides"@en . "Novel Biocatalysts for the Production of Glycosides" . . . "1"^^ . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=7E11011"^^ . "0"^^ .