" protein" . "Byly z\u00EDsk\u00E1ny nov\u00E9 poznatky o regulaci meiotick\u00E9ho zr\u00E1n\u00ED oocyt\u016F na \u00FArovni proteomu, identifikov\u00E1ny proteinov\u00E9 bioznaky lidsk\u00E9 folikul\u00E1rn\u00ED tekutiny a verifikov\u00E1ny potenci\u00E1ln\u00ED prediktivn\u00ED z\u00E1stupce pro in vitro fertilizaci v l\u00E9\u010Db\u011B neplodnosti."@cs . " protein phosphorylation" . . . "Oocyty savc\u016F jsou z\u00E1kladn\u00EDm prvkem pro celou \u0159adu slibn\u00FDch technologi\u00ED, nap\u0159. asistovanou reprodukci (lid\u00ED i zv\u00ED\u0159at), zachov\u00E1n\u00ED genetick\u00E9 diversity, a bun\u011B\u010Dn\u00E9 terapie s vyu\u017Eit\u00EDm kmenov\u00FDch bun\u011Bk. Tyto aplikace kladou zv\u00FD\u0161en\u00FD po\u017Eadavek na dostupnost zral\u00FDch oocyt\u016F se z\u00EDskanou \u00FAplnou v\u00FDvojovou kompetenc\u00ED. Z t\u011Bchto d\u016Fvod\u016F z\u016Fst\u00E1v\u00E1 sledov\u00E1n\u00ED exprese a funkce mnoha gen\u016F a protein\u016F p\u0159edm\u011Btem intenzivn\u00EDho z\u00E1jmu u mnoha \u017Eivo\u010Di\u0161n\u00FDch druh\u016F. Tento projekt c\u00EDlen\u00E9ho v\u00FDzkumu se zam\u011B\u0159uje na : a) studium mechanism\u016F uplat\u0148uj\u00EDc\u00EDch se p\u0159i z\u00EDsk\u00E1v\u00E1n\u00ED meiotick\u00E9 a v\u00FDvojov\u00E9 kompetence oocyt\u016F na hladin\u011B celkov\u00FDch proteinov\u00FDch profil\u016F (proteom\u016F); b) v\u00FDb\u011Br potenci\u00E1ln\u00EDch bioznak\u016F vypov\u00EDdaj\u00EDc\u00EDch o kvalit\u011B zral\u00FDch oocyt\u016F; c) n\u00E1vrh optim\u00E1ln\u00EDch podm\u00EDnek aplikovateln\u00FDch pro \u00FAsp\u011B\u0161nou in vitro produkci embry\u00ED v reproduk\u010Dn\u00ED biotechnologii hospod\u00E1\u0159sk\u00FDch zv\u00ED\u0159at; d) ov\u011B\u0159en\u00ED inova\u010Dn\u00EDch postup\u016F pro jejich \u00FAsp\u011B\u0161nou realizaci v zem\u011Bd\u011Blstv\u00ED." . " meiotic competence" . " proteomics" . . " follicles" . "http://www.isvav.cz/projectDetail.do?rowId=1QS500450568"^^ . " in vitro fertilization" . . . . "oocyte" . . " meiosis" . . "5"^^ . "2010-04-15+02:00"^^ . . "5"^^ . . . " two-step culture" . "2009-03-11+01:00"^^ . . . . . "1"^^ . . . "2"^^ . . " maturation" . . . " developmental competence" . "0"^^ . "oocyte; follicles; meiosis; two-step culture; maturation; meiotic competence; developmental competence; in vitro fertilization; embryo development; protein; proteomics; protein phosphorylation; biomarkers; reproductive biotechnology"@en . " embryo development" . "Female gametes - regulation of their meiotic and developmental competence and possibilities of its practical application"@en . "1QS500450568" . " biomarkers" . "New knowledge about regulation of oocyte meiotic competence at the level of proteome was described. The protein biomarkers of human follicular fluid were identified and potential predictors of in vitro fertilisation were verified for infertility therapy."@en . "2005-07-01+02:00"^^ . "2009-12-31+01:00"^^ . "The mammalian oocyte is the cornerstone for a wide range of biotechnologies such as assisted reproduction (human and animal), preservation of genetic diversity and cell therapies through stem cell technologies.These uses place increased demand for mature oocytes acquiring full developmental competence.For these reasons, the expression and function of a variety genes and proteins implicated in meiotic maturation, oocyte fertilization and embryonal development remain the subject of intensive studies in various species.This aimed research proposal is focused on: a) studies of the mechanisms underlying oocyte meiotic and developmental competence at the level of global protein profiles (proteomes); b) selection of potential biomarkers reflecting the quality of mature oocytes; c) proposal for the optimal conditions allowing succesfull in vitro production of embryos applicable in farm animal reproductive biotechnology; d) validation of inovative approaches to be used in agriculture."@en . "Sami\u010D\u00ED pohlavn\u00ED bu\u0148ky - regulace jejich meiotick\u00E9 a v\u00FDvojov\u00E9 kompetence a mo\u017Enosti jej\u00EDho praktick\u00E9ho vyu\u017Eit\u00ED" .