. "1"^^ . . "2010-04-15+02:00"^^ . "1QS500040508" . . "2009-12-31+01:00"^^ . . "Zjistili jsme, \u017Ee antigenn\u00ED znaky heterochromatinu jako proteiny HP1 a methylovan\u00FD histon H3K9, kter\u00E9 jsou b\u011B\u017En\u00E9 v lidsk\u00FDch lymfocytech a monocytech, nejsou p\u0159\u00EDtomny ve zdrav\u00FDch granulocytech. Monomethylovan\u00FD a zvl\u00E1\u0161t\u011B dimethylovan\u00FD H3K9 se v\u0161ak vyskytuj\u00ED v granulocytech pacient\u016F s chronickou myeloidn\u00ED leuk\u00E9mi\u00ED (CML), bez HP1 protein\u016F. Methylaci H3K9 jsme pozorovali i u pacient\u016F bez typick\u00E9 translokace BCR/ABL. P\u0159\u00EDtomnost methylovan\u00E9ho H3K9 v granulocytech CML nazna\u010Duje ne\u00FAplnou kondenzaci chromatinu, co\u017E m\u016F\u017Ee souviset s prolifera\u010Dn\u00ED kapacitou leukemick\u00FDch bun\u011Bk a m\u016F\u017Ee m\u00EDt v\u00FDznam pro progn\u00F3zu l\u00E9\u010Debn\u00E9ho postupu a mo\u017Enou recidivu choroby. V projektu chceme naj\u00EDt p\u0159\u00ED\u010Dinu p\u0159\u00EDtomnosti methylovan\u00E9ho histonu v granulocytech pacient\u016F s CML, souvislost mezi stupn\u011Bm methylace, zp\u016Fsobem l\u00E9\u010Dby a vymizen\u00EDm klonu s translokac\u00ED BCR/ABL, vypracovat metodu na detekci gen\u016F aktivovan\u00FDch u CML pacient\u016F po odstran\u011Bn\u00ED patogenn\u00EDho klonu pro mo\u017Enost nalezen\u00ED jejich inhibitor\u016F a zabr\u00E1n\u011Bn\u00ED recidiv\u011B." . "Was found that typical characteristic of leukemia cells of CML or AML is the presence of neutrophils with HP1 protein as well as detectable histone HK3K9. Method of detection of a small number of neutrophiles in the blood of patients was elaborated."@en . . "http://www.isvav.cz/projectDetail.do?rowId=1QS500040508"^^ . . . . "23"^^ . "We found that heterochromatin antigenic markers as HP1 proteins and methylated histone H3K9 while present in human lymphocytes and monocytes are absent from healthy granulocytes. Monomethylated and especially dimethylated H3K9 are, however, present in granulocytes of patients with chronic myeloid leukemia (CML) without HP1 proteins. We observed methylation of H3K9 even in patients without typical BCR/ABL translocation. The exposure of methylated H3K9 in CML granulocytes suggests an incomplete chromatin condensation that may be linked to the leukemia cell proliferation capacity and be important for prognosis of the disease treatment and relapse. In the proposed project we wish to find the cause of the methylated histone presence in CMLgranulocytes, the relation between the level of methylation, treatment and elimination of the clone with BCR/ABL translocation, elaborate the method for the detection of genes activated in CML granulocytes after elimination of pathogenic clone."@en . . "23"^^ . . "Zji\u0161t\u011Bno, \u017Ee typickou charakteristikou leukemick\u00FDch bun\u011Bk CML nebo AML je p\u0159\u00EDtomnost neutrofil\u016F s proteinem HP1 a metylovan\u00FDm histonem H3K9 dostupn\u00FDm pro detekci. Nalezena metoda detekce mal\u00E9ho mno\u017Estv\u00ED t\u011Bchto neutrofil\u016F v krvi pacienta po l\u00E9\u010Db\u011B."@cs . . "Methylace histonu H3 v granulocytech jako prognostick\u00FD marker remise chronick\u00E9 myeloidn\u00ED leuk\u00E9mie" . . . . . "Methylation of histone H3 as a prognostic marker of chronic myeloid leukemia remission"@en . " chromatin condensation" . . "2005-01-01+01:00"^^ . "granulocyte differentiation" . "granulocyte differentiation; chromatin condensation; histone methylation; chronic myeloid leukemia; HP1 protein"@en . " histone methylation" . "2009-03-11+01:00"^^ . . . " chronic myeloid leukemia" . . "0"^^ . "0"^^ .