. " monoclonal antibodies" . "140"^^ . . "Center of Molecular and Cellular Immunology"@en . "140"^^ . "Important results on immune functions of signaling molecules were obtained. The results were adequately published in high quality international journals. The major emphasis was on applied research aiming at producing commercializable laboratory research tools and at their transfer to industrial partners. The overall quality of the project was high."@en . "2011-02-03+01:00"^^ . . . . . "adaptor proteins, immunoreceptors, immunomodulators, monoclonal antibodies, recombinant proteins"@en . . . " immunomodulators" . . . "2011-12-31+01:00"^^ . "Projekt dos\u00E1hl v\u00FDznamn\u00FDch v\u00FDsledk\u016F v oblasti studia funkc\u00ED signaliza\u010Dn\u00EDch molekul bun\u011Bk imunitn\u00EDho syst\u00E9mu a jejich poruch. V\u00FDsledky byly publikov\u00E1ny v kvalitn\u00EDch mezin\u00E1rodn\u00EDch \u010Dasopisech. T\u011B\u017Ei\u0161t\u011B pr\u00E1ce spo\u010D\u00EDvalo v aplikovan\u00E9m v\u00FDzkumu sm\u011Brovan\u00E9m na p\u0159\u00EDpravu protil\u00E1tek a rekombinantn\u00EDch protein\u016F s komer\u010Dn\u00EDm potenci\u00E1lem, kter\u00E9 byly p\u0159ed\u00E1v\u00E1ny realiza\u010Dn\u00EDm partner\u016Fm. \u00DArove\u0148 \u0159e\u0161en\u00ED byla vysok\u00E1."@cs . "1M0506" . "1"^^ . "4"^^ . "Elucidate immune functions of selected signaling molecules(adaptor proteins PAG, NTAL, LIME, nonreceptor protein-tyrosine and lipid phosphatases) and defects of their functions in some diseases. Identify, clone and functionally chracterize potential noveClarify signal transduction from apoptosis-ducing receptors of the cytokine TRAIL and receptor DR6 and identify the natural ligand of DR6. Introduce production of recombinant factors Wnt1 and Wnt3a and use them for elucidation of the Wnt-signaling pathwaCharacterize the role of signaling and immunoregulatory molecules (cytokines, bacterial toxins and other natural and synthetic immunomodulators) in activation of macrophages and dendritic cells, e.g. in xenotransplantation reactions (cornea transplantatiConstruct a series of commercially potentially interesting: (a) hybridoma cell lines producing monoclonal antibodies to important known and newly discovered immunocyte signaling proteins; (b) relevant recombinant proteins (biologically active fragments o"@en . . " immunoreceptors" . "2005-01-01+01:00"^^ . "adaptor proteins" . . "0"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=1M0506"^^ . "2012-07-03+02:00"^^ . . "Centrum molekul\u00E1rn\u00ED a bun\u011B\u010Dn\u00E9 imunologie" . "Objasnit imunitn\u00ED funkce n\u011Bkter\u00FDch signaliza\u010Dn\u00EDch molekul imunocyt\u016F (adaptorov\u00E9 proteiny PAG, NTAL, LIME, nereceptorov\u00E9 fosfatasy) a poruchy jejich funkce u n\u011Bkter\u00FDch onemocn\u011Bn\u00ED. Identifikovat, klonovat a funk\u010Dn\u011B charakterizovat potenci\u00E1ln\u00ED nov\u00E9 signalizObjasnit p\u0159enos sign\u00E1lu z apoptosu indukuj\u00EDc\u00EDch receptor\u016F pro cytokin TRAIL a receptoru DR6 a vyhledat ligand pro DR6. Zav\u00E9st produkci rekombinantn\u00EDch faktor\u016F Wnt1 a Wnt3a a vyu\u017E\u00EDt jich pro objasn\u011Bn\u00ED \u00FAlohy Wnt-signaliza\u010Dn\u00ED dr\u00E1hy p\u0159i potla\u010Den\u00ED apoptosyCharakterizovat roli sign\u00E1ln\u00EDch a imunoregula\u010Dn\u00EDch molekul (cytokin\u016F, bakteri\u00E1ln\u00EDch toxin\u016F a dal\u0161\u00EDch p\u0159irozen\u00FDch a syntetick\u00FDch imunomodul\u00E1tor\u016F) p\u0159i aktivaci makrof\u00E1g\u016F a dendritick\u00FDch bun\u011Bk, nap\u0159\u00EDklad p\u0159i xenotransplanta\u010Dn\u00EDch reakc\u00EDch (transplantace rohZkonstruovat s\u00E9rii komer\u010Dn\u011B potenci\u00E1ln\u011B v\u00FDznamn\u00FDch: (a) hybridomov\u00FDch lini\u00ED produkuj\u00EDc\u00EDch monoklon\u00E1ln\u00ED protil\u00E1tky proti d\u016Fle\u017Eit\u00FDm zn\u00E1m\u00FDm a nov\u011B objeven\u00FDm signaliza\u010Dn\u00EDm protein\u016F imunocyt\u016F; (b) relevantn\u00EDch rekombinantn\u00EDch protein\u016F (biologicky aktivn\u00ED frag" . . . . . . .