"2007-06-25+02:00"^^ . . . . . "1"^^ . " mal\u00E9 jadern\u00E9 ribonukleoproteiny" . "http://www.isvav.cz/projectDetail.do?rowId=1K05009"^^ . "retinitis pigmentosa" . . "The project succeeded according to the submission."@en . "0"^^ . "2"^^ . "Pre-mRNA sest\u0159ih je jedn\u00EDm z kl\u00ED\u010Dov\u00FDch krok\u016F genov\u00E9 exprese. Mnoho genetick\u00FDch onemocn\u011Bn\u00ED m\u00E1 z\u00E1klad pr\u00E1v\u011B v mutac\u00EDch ovliv\u0148uj\u00EDc\u00ED pre-mRNA sest\u0159ih. V tomto projektu navrhujeme studovat b\u00EDlkoviny 61K a hPrp3, kter\u00E9 se \u00FA\u010Dastn\u00ED formov\u00E1n\u00ED sest\u0159ihov\u00FDch komplex\u016F a jejich\u017E mutace byly nalezeny u pacient\u016F s autosom\u00E1ln\u011B d\u011Bdi\u010Dnou retinitis pigmentosa. Pomoc\u00ED molekul\u00E1rn\u011B a bun\u011B\u010Dn\u011B biologick\u00FDch technik (RT-PCR, FRAP, FRET, imunoprecipitace) budeme testovat, jak mutace 61K a hPrp3 ovliv\u0148uj\u00ED formovan\u00ED sest\u0159ihov\u00FDch komplex\u016F a pre-mRNA sest\u0159ih." . . "2"^^ . . . . "Anal\u00FDza mutac\u00ED sest\u0159ihov\u00FDch faktor\u016F spojen\u00FDch s d\u011Bdi\u010Dnou retinitis pigmentosa" . "1K05009" . "retinitis pigmentosa, pre-mRNA sest\u0159ih, mal\u00E9 jadern\u00E9 ribonukleoproteiny,"@en . "Analysis of splicing factor mutations connected with the inherited retinitis pigmentosa"@en . . . . "Projekt usp\u011Bl podle zad\u00E1n\u00ED."@cs . . . . . . . " pre-mRNA sest\u0159ih" . . . "0"^^ . "Pre-mRNA splicing is one of the key steps in the regulation of gene expression and mutations influencing pre-mRNA splicing were found in many genetic disorders. In this project, we propose to study two proteins - 61K and hPrp3, which participate in the formation of splicing complexes. Mutations of 61K and hPrp3 were found in patients with autosomal inherited retinitis pigmentosa. Using molecular and cell biology techniques (RT-PCR, FRAP, FRET, imunoprecipitation) we will test how mutations in 61K and hPrp3 influence the formation of splicing complexes and pre-mRNA splicing."@en .