. "Interakce mutantn\u00EDch protein\u016F p53 s genomovou DNA in vitro a in vivo" . . "2007-08-31+02:00"^^ . . "The project produced expecting results. The final publication were ready just to publish, however they have not been published and printed in scientific journals before the end of the project."@en . . "1K04119" . "tumor suppressor protein p53" . . . . "2004-09-01+02:00"^^ . "Gen n\u00E1dorov\u00E9ho supresoru proteinu p53 pat\u0159\u00ED mezi nej\u010Dast\u011Bji mutovan\u00E9 geny v lidsk\u00E9 rakovin\u011B. Tento projekt je zam\u011B\u0159en na z\u00EDsk\u00E1n\u00ED nov\u00FDch poznatk\u016F o p\u0159\u00EDsp\u011Bvku specifick\u00FDch interakc\u00ED mutantn\u00EDch forem p53 s DNA k jeho onkogenn\u00EDm vlastnostem. Interakce vybran\u00FDch mutant\u016F p53 s r\u016Fzn\u00FDmi sekvencemi a strukturami DNA, v\u010Detn\u011B element\u016F uplatnuj\u00EDc\u00ED se p\u0159i stabilit\u011B genomu, regulaci transkripci a replikace, bude charakterizov\u00E1na in vitro a in vivo. P\u0159i \u0159e\u0161en\u00ED bude vyu\u017Eito n\u011Bkolik metodick\u00FDch p\u0159\u00EDstup\u016F: metody detekce interakc\u00ED DNA-protein, po\u010D\u00EDta\u010Dov\u00E1 anal\u00FDza struktury DNA, detekce sekund\u00E1rn\u00EDch struktur DNA pomoc\u00ED chemick\u00FDch a enzymatick\u00FDch technik, elektrochemick\u00E1 anal\u00FDza DNA a b\u00EDlkovin a metody umo\u017E\u0148uj\u00EDc\u00ED anal\u00FDzu exprese gen\u016F. D\u00E1le navrhujeme vyu\u017E\u00EDt modulaci exprese p53 v bun\u011B\u010Dn\u00FDch lini\u00EDch prost\u0159ednictv\u00EDm siRNA a protin\u00E1dorov\u00FDch \u010Dinidel. O\u010Dek\u00E1v\u00E1me, \u017Ee v\u00FDsledky t\u00E9to studie budou vyu\u017Eiteln\u00E9 p\u0159i v\u00FDvoji nov\u00FDch diagnostick\u00FDch a terapeutick\u00FDch p\u0159\u00EDstup\u016F." . "http://www.isvav.cz/projectDetail.do?rowId=1K04119"^^ . . "5"^^ . . "Interactions of mutant p53 with genomic DNA in vitro and in vivo"@en . "5"^^ . . . "2008-05-19+02:00"^^ . "0"^^ . " mutant p53" . . "1"^^ . . . " supercoiled DNA" . " posttranslational modifications" . " protein conformation" . " DNA-protein interactions" . "0"^^ . . "tumor suppressor protein p53, mutant p53, protein conformation, posttranslational modifications, DNA-protein interactions, DNA structure, supercoiled DNA, control of gene expression, cancer"@en . . "2007-02-16+01:00"^^ . " DNA structure" . "The tumor suppressor gene p53 belongs to the most frequently mutated genes in human cancer. This proposal is focused on elucidation of the impacts of specific mutp53 DNA binding activity in its %22gain of function%22 effect. Binding of selected mutp53 to various DNA sequences and structures, inclusing elements involved in genomic stability and control of transcription or replication will be characterized in vitro and in vivo. Several methodic approaches will be utilized: techniques for the detection of DNA-protein interactions, computer analysis of DNA structure, chemical and enzymatic probes, electrochemical analysis of DNA and proteins and techniques for the detection of gene expression. It is further proposed to utilize modulation of p53 expression in cell lines by siRNA and anti-cancer drugs. It is expected that results of this study will be applicable in development of novel diagnostic and therapeutic approaches."@en . " control of gene expression" . . "Projekt p\u0159inesl o\u010Dek\u00E1van\u00E9 v\u00FDsledky. Z\u00E1v\u011Bre\u010Dn\u00E9 publikace byly sice p\u0159ipraveny do tisku, ale dosud nebyly v \u010Dasopisech publikov\u00E1ny."@cs . . . .