. . . "The sensitive method for gliadin and corresponding prolamins of barley and rye determination in gluten-free foodstuffs will be developed.The aim of project will be solved in agreement with experts demands in that field . The pretreatment of the sampleswith complex matrix before ELISA determination will be worked out. The optimized PCR method for wheat detection in gluten-free foodstuffs will be developed. Daily intake of gliadin will be followed in the group of celiac patients. The separated gliadinswill be identified with regard to the toxic peptides content and their biological activity will be studied. ELISA kit interlaboratory testing will be carried out and data for international standardisation will be prepared."@en . . "Safety evalution of foods for gluten-free diet"@en . " p\u0161enice" . . . . . " citliv\u00E1 metoda" . . . . . "28"^^ . "2008-12-31+01:00"^^ . "28"^^ . " bezlepkov\u00E1 dieta" . . "2009-11-04+01:00"^^ . " stanoven\u00ED gliadinu" . "Hodnocen\u00ED bezpe\u010Dnosti potravin pro bezlepkovou dietu" . " bezlepkov\u00E9 potraviny" . "C\u00EDlem bylo ov\u011B\u0159it vhodnost ELISA metoda pro p\u0159esn\u00E9 stanoven\u00ED gliadin\u016F a prolamin\u016F je\u010Dmene a \u017Eita, optimalizovan\u00E1 PCR metoda na d\u016Fkaz p\u0161enice."@cs . . " PCR" . . "The research aim was to verify the ELISA method suitability for precise determination of gliadins and prolamines in barley (Hordeum vulgare) and rye (Secale cereale), optimised PCR method for wheat (Triticum vugare) detection."@en . " proteomick\u00E1 identifikace" . "1B53002" . " izolace DNA" . "2005-02-01+01:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=1B53002"^^ . . "3"^^ . " obsah gliadinu" . "2008-04-21+02:00"^^ . "2"^^ . . "1"^^ . "celiakie" . . . "C\u00EDlem projektu je vyvinout citlivou ELISA metodu pro stanoven\u00ED gliadinu a odpov\u00EDdaj\u00EDc\u00EDch prolamin\u016F je\u010Dmene a \u017Eita v bezlepkov\u00FDch potravin\u00E1ch v souladu s po\u017Eadavky odborn\u00EDk\u016F v t\u00E9to oblasti. Sou\u010Dasn\u011B bude dopracov\u00E1na metodika p\u0159\u00EDpravy vzork\u016F s komplexn\u00ED matric\u00ED p\u0159ed vlastn\u00EDm stanoven\u00ED gliadinu ELISA metodou. Bude vypracov\u00E1na optimalizovan\u00E1 PCR metoda na d\u016Fkaz p\u0161enice v bezlepkov\u00FDch potravin\u00E1ch. Bude sledov\u00E1n denn\u00ED p\u0159\u00EDjem gliadinu u konzument\u016F s bezlepkovou dietou. Budou identifikov\u00E1ny jednotliv\u00E9 gliadinys ohledem na obsah toxick\u00FDch peptid\u016F a studov\u00E1na jejich biologick\u00E1 aktivita.Bude provedeno mezilaboratorn\u00ED testov\u00E1n\u00ED ELISA soupravy a p\u0159ipraveny podklady pro jej\u00ED mezin\u00E1rodn\u00ED standardizaci." . "celiakie; citliv\u00E1 metoda; bezlepkov\u00E9 potraviny; bezlepkov\u00E1 dieta; imunochemick\u00E9 metody; ELISA; PCR; p\u0161enice; izolace DNA; obsah gliadinu; stanoven\u00ED gliadinu; proteomick\u00E1 identifikace; prolaminy je\u010Dmene a \u017Eita"@en . " imunochemick\u00E9 metody" . " ELISA" .