"The aim of the project is to develop complex procedures of diagnostics of economically important and quarantine phytopatogenic organisms for certification system of fruit trees in the Czech Republic. Microbiological,biological and molecular methods ofdetection of bacteria Xylella fastidiosa and serological methods of detection of Phytophthora cactorum will be developed. Nematological laboratory for detection of nematods g.Longidorus and Xiphinema will be established and the presence of nematodes inprimary sources verified. Detection methods of nepoviruses,Myrobalan latent ringspot,Tomato black ringspot,Cherry leaf roll and Strawberry ringspot viruses in cultivars and rootstocks of stone fruits will be devoloped for certification system of fruit trees. The method of reliable detection of apple chlorotic leaf spot virus in cultivars and rootstocks of pears,apple mosaic and apple stem grooving viruses in cultivars and rootstocks of apples will be set up. Molecular,biological and serological metho"@en . "2"^^ . . . . . "40"^^ . . "40"^^ . "C\u00EDlem projektu je v\u00FDzkum a v\u00FDvoj komplexn\u00EDch postup\u016F diagnostiky hospod\u00E1\u0159sky v\u00FDznamn\u00FDch a karant\u00E9nn\u00EDch fytopatogenn\u00EDch organism\u016F pro zaveden\u00ED syst\u00E9mu syst\u00E9m certifikace ovocn\u00FDch d\u0159evin v \u010CR. Pro syst\u00E9m certifikace budou vyvinuty mikrobiologick\u00E9, biologick\u00E9 a molekul\u00E1rn\u00ED metody detekce bakterie Xylella fastidiosa a s\u00E9rologick\u00E9 metody detekce Phytophthora cactorum. Bude etablov\u00E1na nematologick\u00E1 laborato\u0159 pro stanoven\u00ED h\u00E1\u010F\u00E1tek rod\u016F Longidorus a Xiphinema a ov\u011B\u0159ena p\u0159\u00EDtomnost t\u011Bchto h\u00E1\u010F\u00E1tek na prim\u00E1rn\u00EDch zdroj\u00EDch peckovin. Bude vypracov\u00E1na metoda stanoven\u00ED nepovir\u016F: Myrobalan latent ringspot virus, Tomato black ring virus, Cherry leaf roll virus a Strawberry latent ringspot virus v odr\u016Fd\u00E1ch a podno\u017E\u00EDch peckovin pro syst\u00E9m certifikace ovocn\u00FDch d\u0159evin. Bude vyvinuta metoda spolehliv\u00E9ho stanoven\u00ED ACLSV v odr\u016Fd\u00E1ch a podno\u017E\u00EDch hru\u0161n\u011B, ApMV a ASGV v odr\u016Fd\u00E1ch a podno\u017E\u00EDch jablon\u011B. Budou vyvinuty molekul\u00E1rn\u00ED, biologick\u00E9 a s\u00E9rologick\u00E9 metody stanoven\u00ED patogen\u016F cherry mottle leaf virus, peach latent mosaic vir"@cs . . . "Development of complex procedures of diagnostics of economically important and quarantine phytopatogenic organisms for developing of fruit trees certification system in the Czech Republic."@en . "bacteria"@en . "V\u00FDzkum a v\u00FDvoj komplexn\u00EDch postup\u016F diagnostiky hospod\u00E1\u0159sky v\u00FDznamn\u00FDch a karant\u00E9nn\u00EDch fytopatogenn\u00EDch organism\u016F pro syst\u00E9m certifikace ovocn\u00FDch d\u0159evin."@cs . . . "16059"^^ . . "0"^^ . "3"^^ . "16059"^^ .