"0"^^ . "7"^^ . "7"^^ . "Charakteristika kmen\u016F viru reproduk\u010Dn\u00EDho a respira\u010Dn\u00EDho syndromu prasat(PRRS) izolovan\u00FDch v \u010CR."@cs . . . . "1770"^^ . . . . . . . "1"^^ . "1770"^^ . "Characterisation of the strains of porcine reproductive and respiratory syndrome ( PRRS ) isolated in the Czech Republic in 1995-1998 will include tests of potential to replicate in the cell line MARC-145 and investigations of the effect of freeze -drying and long-term storage of freeze-dried strains on infectiosity. The strains will be typified using RT-PCR and direct sequencing of PCR products. Effects of long-term passaging of the strain CAPM V-502 on selected regions of viral genome and pathogenicity for swine will be tested. Monoclonal antibodies to PRRS virus will be prepared and tested for their application in diagnostics and differentiation of virus strains."@en . "Budou charakterizov\u00E1ny kmeny viru reproduk\u010Dn\u00EDho a respira\u010Dn\u00EDho syndromu prasat ( PRRS ) izolovan\u00E9 v \u010Cesk\u00E9 republice v r. 1995-1998.Ov\u011B\u0159ena bude schopnost replikace izol\u00E1t\u016F na bun\u011B\u010Dn\u00E9 linii MARC-145 a vliv lyofilizace a dlouhodob\u00E9ho uchov\u00E1v\u00E1n\u00ED lyofiliz\u00E1t\u016F na infek\u010Dnos viru .Ke genotypizaci izol\u00E1t\u016F bude pou\u017Eita metoda RT-PCR a metoda p\u0159\u00EDm\u00E9 sekvenace PCR produkt\u016F.Bude ov\u011B\u0159en vliv dlouhodob\u00E9ho pas\u00E1\u017Eov\u00E1n\u00ED izol\u00E1tu CAPM V-502 na mo\u017En\u00E9 zm\u011Bny v n\u011Bkter\u00FDch \u00FAsec\u00EDch virov\u00E9ho genomu a na patogenitu izol\u00E1tu pro prasata. Budou p\u0159ipraveny, testov\u00E1ny a srovn\u00E1v\u00E1ny monoklon\u00E1ln\u00ED protil\u00E1tky proti viru PRR a jejich vyu\u017Eitelnost v diagnostice a p\u0159i diferenciaci virov\u00FDch kmen\u016F."@cs . . "virus PRRS, RT-PCR,"@en . "Characterization of porcine reproductive and respiratory syndrome ( PRRS ) virus strains isolated in the Czech Republic"@en . . . "0"^^ .