"8"^^ . . "The aim of the project is to use new methods of molecular genetics in diagnosis of bacterial pathogens in farm animals. The project includes techniques based on PCR for diagnosis of dermonecrotoxin of Pasteurella multocida, hemolysins of Actinobacillus pleuropneumoniae, verotoxin of E.coli, listeriolysin of Listeria monocytogenes and IS900 and IS901 of Mycobacterium paratuberculosis and M.avium, respectively, RAPD PCR for differentiation in mycobacteria and Salmonellae and RFLP PCR in L.monocytogenes. In DNA fingerprinting special attention will be given to replacement of radioactive by non-radioactive systems for labelling DNA probes and to preparation of better defined probes generated by PCR (IS900 and IS901 fingerprinting in mycobacteria and ribotyping and IS200 fingerprinting in Salmonellae). Last part of the project will be elucidation of species specific growth inhibition of bacterial culture in the early stages of stationary phase."@en . "0"^^ . "3375"^^ . "0"^^ . . . . . "1"^^ . "Vyu\u017Eit\u00ED metod molekul\u00E1rn\u00ED genetiky v diagnostice a profylaxi bakteri\u00E1ln\u00EDch chorob hospod\u00E1\u0159sk\u00FDch zv\u00ED\u0159at"@cs . . . "3375"^^ . "Molekul\u00E1rn\u00ED genetika je dynamicky se rozv\u00EDjej\u00EDc\u00ED se obor, kter\u00FD nach\u00E1z\u00ED st\u00E1le v\u011Bt\u0161\u00ED uplatn\u011Bn\u00ED ve veterin\u00E1rn\u00ED i hum\u00E1nn\u00ED medic\u00EDn\u011B. Navrhovan\u00FD \u00FAkol bude zam\u011B\u0159en na studium metod molekul\u00E1rn\u00ED genetiky a na v\u00FDb\u011Br, testov\u00E1n\u00ED a zav\u00E1d\u011Bn\u00ED vhodn\u00FDch metod do diagnostick\u00E9 praxe. D\u016Fraz bude kladen na metody umo\u017E\u0148uj\u00EDc\u00ED rychlej\u0161\u00ED a p\u0159esn\u011Bj\u0161\u00ED diagnostiku a klasifikaci bakteri\u00E1ln\u00EDch patogen\u016F pomoc\u00ED PCR, DNA hybridizace a sekvenov\u00E1n\u00ED a d\u00E1le na metody nahrazuj\u00EDc\u00ED komplikovan\u00E9 testy na \u017Eiv\u00FDch zv\u00ED\u0159atech (dermonekrotoxin Pasteurella multocida). Navrhovan\u00FD projekt bude zahrnovat vyu\u017Eit\u00ED PCR pro detekci patogenn\u00EDch kmen\u016F P. multocida produkuj\u00EDc\u00EDch dermonekrotoxin, identifikaci verotoxinogenn\u00EDch kmen\u016F E.coli, detekci a diferenciaci hemolysin\u016F Actinobacillus pleuropneumoniae,pr\u016Fkaz listeriolysinu Listeria monocytogenes a pr\u016Fkaz sekvence IS900 a IS901 u Mycobacterium paratuberculosis a M.avium. Metoda PCR bude vyu\u017Eita i p\u0159i diferenciaci kmen\u016F L.monocytogenes pomoc\u00ED RFLP PCR a p\u0159i RAPD PCR pro diferenciaci kmen\u016F salmonel a"@cs . "8"^^ . "Molecular genetic methods in diagnosis of bacterial pathogens in farm animals"@en . . . .