. "Nitrofuranov\u00E1 antibiotika jsou veterin\u00E1rn\u00ED l\u00E9\u010Diva, jejich\u017E pou\u017Eit\u00ED v \u017Eivo\u010Disn\u00E9 v\u00FDrob\u011B je vzhledem ke zdravotn\u00EDm rizik\u016Fm v \u010Cesk\u00E9 republice a EU zak\u00E1z\u00E1no. Ned\u00E1vn\u00E9 zji\u0161t\u011Bn\u00ED, \u017Ee nitrofurany jsou st\u00E1le pou\u017E\u00EDv\u00E1ny ve sv\u011Bt\u011B i v n\u011Bkter\u00FDch evropsk\u00FDch zem\u00EDch vyvolalo v t\u00E9to oblasti glob\u00E1ln\u00ED krizi. Analytick\u00E9 metody zaveden\u00E9 ke kontrole nitrofuranov\u00FDch l\u00E9\u010Div (furazolidonu, nitrofurantoinu, furaltadonu a nitrofurazonu) jsou zalo\u017Eeny na detekci metabolit\u016F (3-amino-2-oxazolidinonu (AOZ,1-amino-hydantoinu(AHD,3-amino-5-methylmorpholino-2-oxazolidinonu (AMOZ) a semikarbazidu (SEM), v\u00E1zan\u00FDch na tk\u00E1n\u011B zv\u00ED\u0159at. Dosud zaveden\u00E9 screeningov\u00E9 testy ELISA pro v\u00E1zan\u00E9 metabolity AOZ a AMOZ umo\u017E\u0148uj\u00ED detekci t\u011Bchto analyt\u016F s pou\u017Eit\u00EDm dvou samostatn\u00FDch syst\u00E9m\u016F v komer\u010Dn\u011B dostupn\u00FDch souprav\u00E1ch. C\u00EDlem projektu je vyvinut\u00ED ELISA metody, kter\u00E1 umo\u017En\u00ED sou\u010Dasnou detekci dvou a v\u00EDce nitrofuranov\u00FDch metabolit\u016F v r\u00E1mci jednoho testu (tzv. generick\u00FD test) a t\u00EDm usnadn\u00ED a zrychl\u00ED screeningovou anal\u00FDzu. K dosa\u017Een\u00ED tohoto c\u00EDle je navr\u017Een\u00E1 pro"@cs . "Development of antibodies and screening tests for the determination of residues of nitrofuran antibiotics in foods"@en . "1"^^ . "0"^^ . . . "Nitrofuran antibiotics are veterinary drugs whose use in animal production is banned in the Czech Republic and EU due to health risks. Recent findings that nitrofurans are still used in some European countries and around the world has caused a crisis inthe field. Analytical methods for nitrofuran drug (furazolidone, nitrofurantoin, furaltadone a nitrofurazone) control are based on the detection of tissue-bound metabolites (3-amino-2-oxazolidinone (AOZ),1-amino-hydantoin (AHD), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) a semikarbazide (SEM). Up to now the establisment of screening ELISA tests for bound metabolites AOZ and AMOZ has enabled the detection of these analytes using two single systems (kits).The aim of this project is ELISA development allowing simultaneous detection of two or more nitrofuran metabolites within (one) single test (so called generic test) to simplify and accelerate the screening analysis. To achieve this aim, the suggested development of generic antibodies has been ba"@en . "V\u00FDvoj protil\u00E1tek a screeningov\u00FDch test\u016F pro stanoven\u00ED rezidu\u00ED nitrofuranov\u00FDch antibiotik v potravin\u00E1ch"@cs . . "2008-12-31+01:00"^^ . . . "nitrofurany; semikarbazid; residua; imunoanal\u00FDza; ELISA; generick\u00E9 protil\u00E1tky; ochrana spot\u0159ebitele"@en . "2005-02-01+01:00"^^ . "4868"^^ . . . . "1"^^ . . . "6"^^ . "4868"^^ . . "6"^^ .