. "1"^^ . . . "U pacient\u016F s CVID, IgAD a IgMD budeme s vyu\u017Eit\u00EDm Ig-specifick\u00E9ho RT-PCR na \u00FArovni jedn\u00E9 bu\u0148ky analyzovat somatick\u00E9 hypermutace, zn\u00E1mky afinitn\u00ED maturace, a izotypov\u00E9ho p\u0159esmyku, schopnost proliferace a aktivace, stav BCR signalizace a diferencia\u010Dn\u00ED stadium v jednotliv\u00FDch B lymfocyt\u00E1rn\u00EDch subpopulac\u00ED p\u0159ed a po stimulaci IL-4, IL-21 a anti-CD40 v kombinaci s tetanov\u00FDm toxoidem a pnemokokov\u00FDm sacharidem. Bude vytvo\u0159ena banka EBV-transformovan\u00FDch B-lymfocyt\u00E1rn\u00EDch lini s c\u00EDlem stanovit pod\u00EDl B lymfocyt\u016F v patogenezi uveden\u00FDch chorob. O\u010Dek\u00E1van\u00FDmi v\u00FDstupy projektu jsou publikace v impaktovan\u00E9m zahrani\u010Dn\u00EDm v\u011Bdeck\u00E9m \u010Dasopise, dopln\u011Bn\u00ED znalosti etiopatogeneze CVID a IgAD/IgMD, definov\u00E1n\u00ED diferencia\u010Dn\u00EDho stadia studovan\u00FDch subpopulac\u00ED a kvality BCR signalizace, definice nov\u00FDch klasifika\u010Dn\u00EDch krit\u00E9ri\u00ED s c\u00EDlem zlep\u0161en\u00ED diagnostiky a terapie ve smyslu nastaven\u00ED konkr\u00E9tn\u00ED individu\u00E1ln\u00ED imunoterapie pacient\u016F (v souladu s prioritami Resortn\u00EDho programu v\u00FDzkumu a v\u00FDvoje MZ \u010CR)."@cs . "CVID; IgA deficiency; IgM deficiency; development of B-cells; flow cytometry"@en . "Poruchy diferenciace B lymfocyt\u016F u pacient\u016F s defekty humor\u00E1ln\u00ED imunity"@cs . "1"^^ . . . "The Ig-specific single-cell RT-PCR in B cells of patients with CVID, IgAD/IgMD will be used to analyze somatic hypermutation, affinity maturation/isotype switch, proliferation and activation, BCR signalling and differentiation stadium of B cell subsets before and after stimulation with IL-4, IL-21 and anti-CD40 together with tetanus toxoid/pneumococcal saccharide. The bank of EBV-transformed B-lymphocyte lines will be generated to address their contribution to the pathogenesis of mentioned diseases. The publication in peer-reviewed scientific journals, extension of knowledge on pathogenesis of these diseases, determination of differentiation stadium and BCR signalling in B cell subsets and new classification diagnostic criteria represent anticipated outputs. The improvement of diagnosis/ therapy leading to the selection of particular individual immunotherapy is the main focus of the study in accordance the priorites of declared \u201EProgramme of Research and Development of the Czech Ministry of Health\u201C."@en . "Disturbances of differentiation of B-lymphocytes in patients with\u00A0defects of humoral immunity"@en . "2014-12-31+01:00"^^ . "2"^^ . "1"^^ . . . "10254"^^ . . . "2010-09-01+01:00"^^ . . "10254"^^ . "0"^^ .