. "2009-01-01+01:00"^^ . "0"^^ . . "1"^^ . "2011-12-31+01:00"^^ . "3034"^^ . . . "8"^^ . "Projekt je zam\u011B\u0159en na vy\u0161et\u0159en\u00ED n\u011Bkolika aspekt\u016F selektivn\u00EDho deficitu IgA(IgAD). Bude sledov\u00E1n v\u00FDskyt mutac\u00ED genu TACI u osob s IgAD a v b\u011B\u017En\u00E9 populaci. A\u010Dkoliv jsou mutace TACI \u010Dast\u011Bj\u0161\u00ED u osob s geneticky p\u0159\u00EDbuzn\u00FDm b\u011B\u017En\u00FDm variabiln\u00EDm imunodeficitem, zat\u00EDm jedin\u00E1 rozs\u00E1hl\u00E1 studie proveden\u00E1 na ne-slovansk\u00E9 populaci asociaci s IgAD nepotvrdila. D\u00E1le budeme sledovat lymfocyt\u00E1rn\u00ED subpopulace, jejich\u017E po\u010Det byl zv\u00FD\u0161en v na\u0161ich p\u0159edchoz\u00EDch studi\u00EDch. Bude se jednat zejm\u00E9na o subpopulaci CD4+CD25+. M\u016F\u017Ee se jednat o bu\u0148ky aktivovan\u00E9, ale i Treg lymfocyty, ty budeme detekovat pomoc\u00ED kombinace znak\u016F CD4,CD25,CD127. Bude zkoum\u00E1na i subpopulace B-lymfocyt\u016F CD21-CD38-, jej\u00ED\u017E procentu\u00E1ln\u00ED zastoupen\u00ED bylo u nemocn\u00FDch s IgAD zv\u00FD\u0161eno. Soust\u0159ed\u00EDme se na fenotypovou charakteristiku t\u011Bchto bun\u011Bk a z\u00E1vislost na p\u0159\u00EDtomnosti autoimunitn\u00EDch fenom\u00E9n\u016F. Lymfocyt\u00E1rn\u00ED abnormality budou korelov\u00E1ny s hladinou endotoxin\u00E9mie ke zji\u0161t\u011Bn\u00ED, zda-li zv\u00FD\u0161en\u00E1 resorpce endotoxinu nevysv\u011Btluje zm\u00EDn\u011Bn\u00E9 abnormality aktivac\u00ED imunitn\u00EDho syst\u00E9mu."@cs . . "8"^^ . . . . . "IgA deficiency; lymphocyte subsets; differentiation antigens; mutation analysis"@en . "3034"^^ . . "1"^^ . "V\u00FDznam genu TACI a abnormalit lymfocyt\u00E1rn\u00EDch subpopulac\u00ED v patogenezi selektivn\u00EDho deficitu IgA"@cs . "The project is focused on selective IgA deficiency (IgAD). The frequency of TACI mutations will be determined in IgAD patients and in a healthy Czech population. Although the TACI mutations were shown to be increased in patients with genetically related CVID, the only study published yet did not find any increase in TACI mutation in IgAD patients. The lymphocyte subpopulations which were shown to be abnormal in IgAD patients in our previous studies will also be studied. These will include CD4+CD25+ cells, which can both be activated such as regulatory (Treg) lymphocytes. The number of Treg cells will be determined by a combination of CD4,CD25,CD127 antigens. CD21-CD38- B-cells will also be studied by immunophenotype analysis and correlated with autoimmune phenomena in IgAD patients. Lymphocyte subpopulations will also be correlated with the serum endotoxin level to elucidate whether increased endotoxin resorption may explain the above-mentioned abnormalities by activation of the immune system."@en . "Significance of TACI mutations and lymphocyte subpopulations in pathogenesis of selective IgA deficiency"@en .