. . . "Development of a standardized methodology for the collection and exact analysis of exhaled breath condensate with the emphasis on the reliable recording of a number of parameters potentially affecting the validity of the acquired concentration levels of arachidonic acid derivatives (leukotriene \u2013 LTB4, cysteinyl LTC4, LTD4, LTE4, 8-isoprostane). Within the framework of the project, optimal technical and analytical conditions will be assessed during the determinations of LT in different day and year periods, different climatic conditions and environmental air pollution. Markers will be studied both in healthy volunteers and subjects with allergic diseases (bronchial asthma, allergic rhinitis). Analytic methodology will consist of biomarker isolation from a complex biological matrix, followed by a highly selective and sensitive detection method based on mass spectrometry, enabling to identify and quantify biomarkers at the concentration levels in picograms in a milliliter of the biological matrix."@en . "6609"^^ . . . "10"^^ . "Vyvinut\u00ED standardizovan\u00E9 metodiky odb\u011Bru a anal\u00FDzy kondenz\u00E1tu vydechovan\u00E9ho vzduchu a mo\u010De s d\u016Frazem na studium \u0159ady parametr\u016F, kter\u00E9 mohou ovlivnit validitu marker\u016F ze skupiny metabolit\u016F arachidonov\u00E9 kyseliny (8-isoprostan, leukotrien LTB4, cysteinylov\u00E9 leukotrieny LTC4, LTD4, LTE4). V r\u00E1mci studie budou neinvazivn\u00ED metodou p\u0159i stanoven\u00ED marker\u016F v r\u016Fzn\u00FDch denn\u00EDch i ro\u010Dn\u00EDch obdob\u00EDch, za r\u016Fzn\u00FDch klimatick\u00FDch podm\u00EDnek i kontaminace venkovn\u00EDho ovzdu\u0161\u00ED u zdrav\u00FDch dobrovoln\u00EDk\u016F a osob s alergick\u00FDmi nemocemi (astma bronchiale, alergick\u00E1 r\u00FDma) zji\u0161\u0165ov\u00E1ny optim\u00E1ln\u00ED technick\u00E9 i analytick\u00E9 podm\u00EDnky, zaru\u010Duj\u00EDc\u00ED standardizaci z\u00EDskan\u00FDch v\u00FDsledk\u016F. Analytick\u00E1 \u010D\u00E1st bude vyu\u017E\u00EDvat kombinace izolace biomarkeru z komplexn\u00ED biologick\u00E9 matrice, p\u0159\u00EDpadn\u011B jeho zkoncentrov\u00E1n\u00ED, s n\u00E1slednou vysoce selektivn\u00ED a citlivou detek\u010Dn\u00ED metodou na principu hmotnostn\u00ED spektrometrie, kter\u00E1 dovoluje identifikovat a kvantifikovat biomarkery v koncentrac\u00EDch na \u00FArovni pikogram\u016F v mililitru biologick\u00E9 matrice."@cs . "2011-12-31+01:00"^^ . "condensate; leukotrienes; 8-isoprostane; analysis; asthma; allergic rhinitis; variability; diagnostic; environmental"@en . "10"^^ . "6609"^^ . "2009-01-01+01:00"^^ . . "Monitorov\u00E1n\u00ED biorytm\u016F metabolit\u016F kyseliny arachidonov\u00E9 standardizovanou metodikou"@cs . . . . "0"^^ . . "3"^^ . . "Monitoring of biorhythms of metabolites arachidonic acid by standardized methodology"@en . "1"^^ . .