"The aim of this project is complex analysis of tumor suppressor p53 in tumor tissue of patients with diffuse large B-cell lymphoma and mantle cell lymphoma to verify whether p53 abberation is useful prognostic marker of the diseases. For detection of p53 abberations, we will employ funtional analysis of separated alleles in yeast (FASAY), DNA sequencing, analysis of the p53 protein level by immunohistochemistry and immunoblotting, and analysis of the locus 17p13 by FISH."@en . . . "9"^^ . . . "9"^^ . "Complex analysis of p53 tumor suppressor in non-Hodgkin\u00B4s lymphomas"@en . "2494"^^ . . . "2009-12-31+01:00"^^ . "non-Hodgkin\u00B4s lymphoma; diffuse large B-cell lymphoma; mantle cell lymphoma; tumor suppressor p53; FASAY; FISH; immunohistochemistry; tumor suppressor ATM"@en . "V p\u0159edlo\u017Een\u00E9m projektu navrhujeme komplexn\u011B vy\u0161et\u0159it n\u00E1dorov\u00FD supresor p53 v n\u00E1dorov\u00E9 tk\u00E1ni pacient\u016F s MCL a DLBCL s c\u00EDlem zjistit, zda je aberace genu p53 vyu\u017Eiteln\u00FDm prognostick\u00FDm markerem tohoto onemocn\u011Bn\u00ED. K detekci aberac\u00ED p53 bude vyu\u017Eita funk\u010Dn\u00ED anal\u00FDza separovan\u00FDch alel v kvasink\u00E1ch, sekvenov\u00E1n\u00ED DNA, anal\u00FDza hladiny proteinu p53 imunohiostochemicky a imunoblotingem a anal\u00FDza delece lokusu 17p13 pomoc\u00ED FISH."@cs . . . "0"^^ . "0"^^ . "1"^^ . "2007-01-01+01:00"^^ . "2494"^^ . . . . "Komplexn\u00ED anal\u00FDza n\u00E1dorov\u00E9ho supresoru p53 u nehodgkinsk\u00FDch lymfom\u016F"@cs .